Emergence and clonal expansion of Vibrio aestuarianus lineages pathogenic for oystersin Europe

• Published in: Molecular Ecology
• Main Authors: Mesnil, Aurélie, Jacquot, Maude, Garcia, Céline, Tourbiez, Delphine, Canier, Lydie, Bidois, Audrey, Dégremont, Lionel, Cheslett, Deborah, Geary, Michelle, Vetri, Alessia, Roque, Ana, Furones, Dolors, Garden, Alison, Orozova, Petya, Arzul, Isabelle, Sicard, Mathieu, Charrière, Guillaume, M, Destoumieux-Garzon, Delphine, Travers, Marie-Agnes
• Other Authors: Interactions Hôtes-Pathogènes-Environnements (IHPE), Université de Perpignan Via Domitia (UPVD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Unité Adaptation et Santé des Invertébrés Marins (ASIM), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Laboratoire de Génétique et Pathologie des Mollusques Marins (LGPMM), Santé, Génétique et Microbiologie des Mollusques (SGMM), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Marine Institute Oranmore, Institut de Recerca i Tecnologia Agroalimentàries = Institute of Agrifood Research and Technology (IRTA), National Diagnostic and Research Veterinary Medical Institute (NDRVMI), Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de recherche pour le développement IRD : UR226-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2023
• Subjects: [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health; [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology; Crassostrea gigas
• Online Access: https://hal.science/hal-04013412; https://hal.science/hal-04013412/document; https://hal.science/hal-04013412/file/Mesnil-2023-MolEcol-Emergence-MANUSCRIT.pdf; https://doi.org/10.1111/mec.16910

International audience Crassostrea gigas oysters represent a significant global food source, with 4.7 million tons harvested per year. In 2001, the bacterium Vibrio aestuarianus francensis emerged as a pathogen that causes adult oyster mortality in France and Ireland. Its impact on oyster aquaculture has increased in Europe since its reemergence in 2012. To better understand the evolutionary mechanisms leading to the emergence and persistence over time of this pathogen, we conducted a survey of mollusk diseases through national reference laboratories (NRLs) across Europe. We analyzed54 new genomes of Vibrio Aestuarianus (Va) isolated from multiple environmental compartments since 2001, in areas with and withoutbivalve mortalities. We used a combination of comparative genomics and population genetics approaches and show thatVa has a classical epidemic population structure from which the pathogenic Va francensis subspecies emerged and clonally expanded. Furthermore, we identified a specific cus-cop-containing island conferring copper resistance to Va francensis whose acquisition may have favored the emergence of pathogenic lineages adapted and specialized to oysters.