Dietary Supplementation with Vitamin K Affects Transcriptome and Proteome of Senegalese Sole, Improving Larval Performance and Quality
Nutritional factors strongly influence fish larval development and skeletogenesis, and may induce skeletal deformities. Vitamin K (VK) has been largely disregarded in aquaculture nutrition, despite its important roles in bone metabolism, in gamma-carboxylation of Gla proteins, and in regulating gene...
Published in: | Marine Biotechnology |
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Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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2014
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Online Access: | https://orbit.dtu.dk/en/publications/eb61df5e-2a82-4ad8-b266-756cc06b7946 https://doi.org/10.1007/s10126-014-9571-2 |
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ftdtupubl:oai:pure.atira.dk:publications/eb61df5e-2a82-4ad8-b266-756cc06b7946 2024-06-23T07:51:25+00:00 Dietary Supplementation with Vitamin K Affects Transcriptome and Proteome of Senegalese Sole, Improving Larval Performance and Quality Richard, Nadege Fernandez, Ignacio Wulff, Tune Hamre, Kristin Cancela, Leonor Conceicao, Luis E. C. Gavaia, Paulo J. 2014 https://orbit.dtu.dk/en/publications/eb61df5e-2a82-4ad8-b266-756cc06b7946 https://doi.org/10.1007/s10126-014-9571-2 eng eng https://orbit.dtu.dk/en/publications/eb61df5e-2a82-4ad8-b266-756cc06b7946 info:eu-repo/semantics/closedAccess Richard , N , Fernandez , I , Wulff , T , Hamre , K , Cancela , L , Conceicao , L E C & Gavaia , P J 2014 , ' Dietary Supplementation with Vitamin K Affects Transcriptome and Proteome of Senegalese Sole, Improving Larval Performance and Quality ' , Marine Biotechnology , vol. 16 , no. 5 , pp. 522-537 . https://doi.org/10.1007/s10126-014-9571-2 BIOTECHNOLOGY MARINE SALMO-SALAR L NICOTINAMIDE BISULFITE VITAMIN-K-3 MUMMICHOG FUNDULUS-HETEROCLITUS XENOBIOTIC RECEPTOR SXR HEAVY-CHAIN GENES GLA-RICH PROTEIN REAL-TIME PCR ATLANTIC SALMON MOLECULAR CHARACTERIZATION VASCULAR CALCIFICATION Vitamin K Skeletal deformities Proteomics Gene expression Solea senegalensis Flatfish larvae HASH(0x3cc4408) /dk/atira/pure/sustainabledevelopmentgoals/life_below_water name=SDG 14 - Life Below Water article 2014 ftdtupubl https://doi.org/10.1007/s10126-014-9571-2 2024-06-04T15:10:52Z Nutritional factors strongly influence fish larval development and skeletogenesis, and may induce skeletal deformities. Vitamin K (VK) has been largely disregarded in aquaculture nutrition, despite its important roles in bone metabolism, in gamma-carboxylation of Gla proteins, and in regulating gene expression through the pregnane X receptor (Pxr). Since the mechanisms mediating VK effects over skeletal development are poorly known, we investigated the effects of VK-supplementation on skeletal development in Senegalese sole larvae, aiming to identify molecular pathways involved. Larvae were fed live preys enriched with graded levels of phylloquinone (PK) (0, 50, and 250 mg kg(-1)) and survival rate, growth, VK contents, calcium content and incidence of skeletal deformities were determined, revealing an improvement of larval performance and decreasing the incidence of deformities in VK-supplemented groups. Comparative proteome analysis revealed a number of differentially expressed proteins between Control and Diet 250 associated with key biological processes including skin, muscle, and bone development. Expression analysis showed that genes encoding proteins related to the VK cycle (ggcx, vkor), VK nuclear receptor (pxr), and VK-dependent proteins (VKDPs; oc1 and grp), were differentially expressed. This study highlights the potential benefits of increasing dietary VK levels in larval diets, and brings new insights on the mechanisms mediating the positive effects observed on larval performance and skeletal development. Article in Journal/Newspaper Atlantic salmon Salmo salar Technical University of Denmark: DTU Orbit Marine Biotechnology 16 5 522 537 |
institution |
Open Polar |
collection |
Technical University of Denmark: DTU Orbit |
op_collection_id |
ftdtupubl |
language |
English |
topic |
BIOTECHNOLOGY MARINE SALMO-SALAR L NICOTINAMIDE BISULFITE VITAMIN-K-3 MUMMICHOG FUNDULUS-HETEROCLITUS XENOBIOTIC RECEPTOR SXR HEAVY-CHAIN GENES GLA-RICH PROTEIN REAL-TIME PCR ATLANTIC SALMON MOLECULAR CHARACTERIZATION VASCULAR CALCIFICATION Vitamin K Skeletal deformities Proteomics Gene expression Solea senegalensis Flatfish larvae HASH(0x3cc4408) /dk/atira/pure/sustainabledevelopmentgoals/life_below_water name=SDG 14 - Life Below Water |
spellingShingle |
BIOTECHNOLOGY MARINE SALMO-SALAR L NICOTINAMIDE BISULFITE VITAMIN-K-3 MUMMICHOG FUNDULUS-HETEROCLITUS XENOBIOTIC RECEPTOR SXR HEAVY-CHAIN GENES GLA-RICH PROTEIN REAL-TIME PCR ATLANTIC SALMON MOLECULAR CHARACTERIZATION VASCULAR CALCIFICATION Vitamin K Skeletal deformities Proteomics Gene expression Solea senegalensis Flatfish larvae HASH(0x3cc4408) /dk/atira/pure/sustainabledevelopmentgoals/life_below_water name=SDG 14 - Life Below Water Richard, Nadege Fernandez, Ignacio Wulff, Tune Hamre, Kristin Cancela, Leonor Conceicao, Luis E. C. Gavaia, Paulo J. Dietary Supplementation with Vitamin K Affects Transcriptome and Proteome of Senegalese Sole, Improving Larval Performance and Quality |
topic_facet |
BIOTECHNOLOGY MARINE SALMO-SALAR L NICOTINAMIDE BISULFITE VITAMIN-K-3 MUMMICHOG FUNDULUS-HETEROCLITUS XENOBIOTIC RECEPTOR SXR HEAVY-CHAIN GENES GLA-RICH PROTEIN REAL-TIME PCR ATLANTIC SALMON MOLECULAR CHARACTERIZATION VASCULAR CALCIFICATION Vitamin K Skeletal deformities Proteomics Gene expression Solea senegalensis Flatfish larvae HASH(0x3cc4408) /dk/atira/pure/sustainabledevelopmentgoals/life_below_water name=SDG 14 - Life Below Water |
description |
Nutritional factors strongly influence fish larval development and skeletogenesis, and may induce skeletal deformities. Vitamin K (VK) has been largely disregarded in aquaculture nutrition, despite its important roles in bone metabolism, in gamma-carboxylation of Gla proteins, and in regulating gene expression through the pregnane X receptor (Pxr). Since the mechanisms mediating VK effects over skeletal development are poorly known, we investigated the effects of VK-supplementation on skeletal development in Senegalese sole larvae, aiming to identify molecular pathways involved. Larvae were fed live preys enriched with graded levels of phylloquinone (PK) (0, 50, and 250 mg kg(-1)) and survival rate, growth, VK contents, calcium content and incidence of skeletal deformities were determined, revealing an improvement of larval performance and decreasing the incidence of deformities in VK-supplemented groups. Comparative proteome analysis revealed a number of differentially expressed proteins between Control and Diet 250 associated with key biological processes including skin, muscle, and bone development. Expression analysis showed that genes encoding proteins related to the VK cycle (ggcx, vkor), VK nuclear receptor (pxr), and VK-dependent proteins (VKDPs; oc1 and grp), were differentially expressed. This study highlights the potential benefits of increasing dietary VK levels in larval diets, and brings new insights on the mechanisms mediating the positive effects observed on larval performance and skeletal development. |
format |
Article in Journal/Newspaper |
author |
Richard, Nadege Fernandez, Ignacio Wulff, Tune Hamre, Kristin Cancela, Leonor Conceicao, Luis E. C. Gavaia, Paulo J. |
author_facet |
Richard, Nadege Fernandez, Ignacio Wulff, Tune Hamre, Kristin Cancela, Leonor Conceicao, Luis E. C. Gavaia, Paulo J. |
author_sort |
Richard, Nadege |
title |
Dietary Supplementation with Vitamin K Affects Transcriptome and Proteome of Senegalese Sole, Improving Larval Performance and Quality |
title_short |
Dietary Supplementation with Vitamin K Affects Transcriptome and Proteome of Senegalese Sole, Improving Larval Performance and Quality |
title_full |
Dietary Supplementation with Vitamin K Affects Transcriptome and Proteome of Senegalese Sole, Improving Larval Performance and Quality |
title_fullStr |
Dietary Supplementation with Vitamin K Affects Transcriptome and Proteome of Senegalese Sole, Improving Larval Performance and Quality |
title_full_unstemmed |
Dietary Supplementation with Vitamin K Affects Transcriptome and Proteome of Senegalese Sole, Improving Larval Performance and Quality |
title_sort |
dietary supplementation with vitamin k affects transcriptome and proteome of senegalese sole, improving larval performance and quality |
publishDate |
2014 |
url |
https://orbit.dtu.dk/en/publications/eb61df5e-2a82-4ad8-b266-756cc06b7946 https://doi.org/10.1007/s10126-014-9571-2 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Richard , N , Fernandez , I , Wulff , T , Hamre , K , Cancela , L , Conceicao , L E C & Gavaia , P J 2014 , ' Dietary Supplementation with Vitamin K Affects Transcriptome and Proteome of Senegalese Sole, Improving Larval Performance and Quality ' , Marine Biotechnology , vol. 16 , no. 5 , pp. 522-537 . https://doi.org/10.1007/s10126-014-9571-2 |
op_relation |
https://orbit.dtu.dk/en/publications/eb61df5e-2a82-4ad8-b266-756cc06b7946 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1007/s10126-014-9571-2 |
container_title |
Marine Biotechnology |
container_volume |
16 |
container_issue |
5 |
container_start_page |
522 |
op_container_end_page |
537 |
_version_ |
1802642512857268224 |