Dietary Supplementation with Vitamin K Affects Transcriptome and Proteome of Senegalese Sole, Improving Larval Performance and Quality

Nutritional factors strongly influence fish larval development and skeletogenesis, and may induce skeletal deformities. Vitamin K (VK) has been largely disregarded in aquaculture nutrition, despite its important roles in bone metabolism, in gamma-carboxylation of Gla proteins, and in regulating gene...

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Bibliographic Details
Published in:Marine Biotechnology
Main Authors: Richard, Nadege, Fernandez, Ignacio, Wulff, Tune, Hamre, Kristin, Cancela, Leonor, Conceicao, Luis E. C., Gavaia, Paulo J.
Format: Article in Journal/Newspaper
Language:English
Published: 2014
Subjects:
BIOTECHNOLOGY
MARINE
SALMO-SALAR L
NICOTINAMIDE BISULFITE VITAMIN-K-3
MUMMICHOG FUNDULUS-HETEROCLITUS
XENOBIOTIC RECEPTOR SXR
HEAVY-CHAIN GENES
GLA-RICH PROTEIN
REAL-TIME PCR
ATLANTIC SALMON
MOLECULAR CHARACTERIZATION
VASCULAR CALCIFICATION
Vitamin K
Skeletal deformities
Proteomics
Gene expression
Solea senegalensis
Flatfish larvae
HASH(0x3cc4408)
/dk/atira/pure/sustainabledevelopmentgoals/life_below_water
name=SDG 14 - Life Below Water
Atlantic salmon
Salmo salar
Online Access:https://orbit.dtu.dk/en/publications/eb61df5e-2a82-4ad8-b266-756cc06b7946
https://doi.org/10.1007/s10126-014-9571-2
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Summary:Nutritional factors strongly influence fish larval development and skeletogenesis, and may induce skeletal deformities. Vitamin K (VK) has been largely disregarded in aquaculture nutrition, despite its important roles in bone metabolism, in gamma-carboxylation of Gla proteins, and in regulating gene expression through the pregnane X receptor (Pxr). Since the mechanisms mediating VK effects over skeletal development are poorly known, we investigated the effects of VK-supplementation on skeletal development in Senegalese sole larvae, aiming to identify molecular pathways involved. Larvae were fed live preys enriched with graded levels of phylloquinone (PK) (0, 50, and 250 mg kg(-1)) and survival rate, growth, VK contents, calcium content and incidence of skeletal deformities were determined, revealing an improvement of larval performance and decreasing the incidence of deformities in VK-supplemented groups. Comparative proteome analysis revealed a number of differentially expressed proteins between Control and Diet 250 associated with key biological processes including skin, muscle, and bone development. Expression analysis showed that genes encoding proteins related to the VK cycle (ggcx, vkor), VK nuclear receptor (pxr), and VK-dependent proteins (VKDPs; oc1 and grp), were differentially expressed. This study highlights the potential benefits of increasing dietary VK levels in larval diets, and brings new insights on the mechanisms mediating the positive effects observed on larval performance and skeletal development.

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