Improved primer sequences for the mitochondrial ND1, ND3/4 and ND5/6 segments in salmonid fishes : application to RFLP analysis of Atlantic salmon
New specific primers for the mtDNA segments ND1, ND3/4 and ND5/6 designed from the rainbow trout sequence, improved PCR amplification for salmonid fishes. RFLP analysis revealed restriction site variation for all three segments in Atlantic salmon. Eleven haplotypes were detected in a screening of 30...
Published in: | Journal of Fish Biology |
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Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
1998
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Subjects: | |
Online Access: | https://orbit.dtu.dk/en/publications/e8b912fc-e394-428b-8f13-9a9c1f1ac036 https://doi.org/10.1006/jfbi.1998.0677 |
Summary: | New specific primers for the mtDNA segments ND1, ND3/4 and ND5/6 designed from the rainbow trout sequence, improved PCR amplification for salmonid fishes. RFLP analysis revealed restriction site variation for all three segments in Atlantic salmon. Eleven haplotypes were detected in a screening of 30 individuals from four European populations. (C) 1998 The Fisheries society of the British Isles. |
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