Improved primer sequences for the mitochondrial ND1, ND3/4 and ND5/6 segments in salmonid fishes : application to RFLP analysis of Atlantic salmon

New specific primers for the mtDNA segments ND1, ND3/4 and ND5/6 designed from the rainbow trout sequence, improved PCR amplification for salmonid fishes. RFLP analysis revealed restriction site variation for all three segments in Atlantic salmon. Eleven haplotypes were detected in a screening of 30...

Full description

Bibliographic Details
Published in:Journal of Fish Biology
Main Authors: Eg Nielsen, Einar, Hansen, Michael Møller, Mensberg, Karen-Lise Dons
Format: Article in Journal/Newspaper
Language:English
Published: 1998
Subjects:
Atlantic salmon
Online Access:https://orbit.dtu.dk/en/publications/e8b912fc-e394-428b-8f13-9a9c1f1ac036
https://doi.org/10.1006/jfbi.1998.0677
Description
Summary:New specific primers for the mtDNA segments ND1, ND3/4 and ND5/6 designed from the rainbow trout sequence, improved PCR amplification for salmonid fishes. RFLP analysis revealed restriction site variation for all three segments in Atlantic salmon. Eleven haplotypes were detected in a screening of 30 individuals from four European populations. (C) 1998 The Fisheries society of the British Isles.

Similar Items