Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion
Bioactivity of cod (Gadus morhua) and chicken (Gallus domesticus) protein hydrolysates before and after in vitro gastrointestinal (GI) digestion was investigated using yeast Saccharomyces cerevisiae as a model organism. Both hydrolysates were exposed to in vitro GI digestion prior to cellular exposu...
Published in: | Food Technology and Biotechnology |
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Language: | English |
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2017
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Online Access: | https://orbit.dtu.dk/en/publications/948e0154-af8b-43f9-9ea2-ef6ab82e6ffa https://doi.org/10.17113/ftb.55.03.17.5117 https://backend.orbit.dtu.dk/ws/files/134357020/Bioactivity_of_Cod_and_Chicken_Protein_Hydrolysates_before_and_after_in_vitro_Gastrointestinal_Digestion.pdf https://backend.orbit.dtu.dk/ws/files/138285503/ftb_55_360.pdf |
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ftdtupubl:oai:pure.atira.dk:publications/948e0154-af8b-43f9-9ea2-ef6ab82e6ffa 2023-05-15T16:19:16+02:00 Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion Jamnik, Polona Istenič, Katja Wulff, Tune Geirsdottir, Margret Almgren, Annette Jonsdottir, Rosa Kristinsson, Hordur Undeland, Ingrid 2017 application/pdf https://orbit.dtu.dk/en/publications/948e0154-af8b-43f9-9ea2-ef6ab82e6ffa https://doi.org/10.17113/ftb.55.03.17.5117 https://backend.orbit.dtu.dk/ws/files/134357020/Bioactivity_of_Cod_and_Chicken_Protein_Hydrolysates_before_and_after_in_vitro_Gastrointestinal_Digestion.pdf https://backend.orbit.dtu.dk/ws/files/138285503/ftb_55_360.pdf eng eng info:eu-repo/semantics/openAccess Jamnik , P , Istenič , K , Wulff , T , Geirsdottir , M , Almgren , A , Jonsdottir , R , Kristinsson , H & Undeland , I 2017 , ' Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion ' , Food Technology and Biotechnology , vol. 55 , no. 3 , pp. 360-367 . https://doi.org/10.17113/ftb.55.03.17.5117 Protein hydrolysates Cod Chicken In vitro gastrointestinal digestion Yeast Proteomics article 2017 ftdtupubl https://doi.org/10.17113/ftb.55.03.17.5117 2023-01-12T00:06:45Z Bioactivity of cod (Gadus morhua) and chicken (Gallus domesticus) protein hydrolysates before and after in vitro gastrointestinal (GI) digestion was investigated using yeast Saccharomyces cerevisiae as a model organism. Both hydrolysates were exposed to in vitro GI digestion prior to cellular exposure to simulate digestion conditions in the human body and therefore investigate the role of modulations in the GI tract on the cell response. The effect of digested and undigested hydrolysates on intracellular oxidation, cellular metabolic energy and proteome level was investigated. No difference in the effect on intracellular oxidation activity was obtained between cod and chicken hydrolysates, while higher effect on intracellular oxidation was provided by digested hydrolysates, with relative values of intracellular oxidation of cod of (70.2±0.8) and chicken of (74.5±1.4) % than by undigested ones, where values of cod and chicken were (95.5±1.2) and (90.5±0.7) %, respectively. Neither species nor digestion had any effect on cellular metabolic energy. At proteome level, digested hydrolysates gave again significantly stronger responses than undigested counterparts; cod peptides here also gave somewhat stronger response than chicken peptides. The knowledge of the action of food protein hydrolysates and their digests within live cells, also at proteome level, is important for further validation of their activity in higher eukaryotes to develop new products, such as in this case chicken and cod muscle-derived peptides as functional ingredients. Article in Journal/Newspaper Gadus morhua Technical University of Denmark: DTU Orbit Food Technology and Biotechnology 55 3 |
institution |
Open Polar |
collection |
Technical University of Denmark: DTU Orbit |
op_collection_id |
ftdtupubl |
language |
English |
topic |
Protein hydrolysates Cod Chicken In vitro gastrointestinal digestion Yeast Proteomics |
spellingShingle |
Protein hydrolysates Cod Chicken In vitro gastrointestinal digestion Yeast Proteomics Jamnik, Polona Istenič, Katja Wulff, Tune Geirsdottir, Margret Almgren, Annette Jonsdottir, Rosa Kristinsson, Hordur Undeland, Ingrid Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion |
topic_facet |
Protein hydrolysates Cod Chicken In vitro gastrointestinal digestion Yeast Proteomics |
description |
Bioactivity of cod (Gadus morhua) and chicken (Gallus domesticus) protein hydrolysates before and after in vitro gastrointestinal (GI) digestion was investigated using yeast Saccharomyces cerevisiae as a model organism. Both hydrolysates were exposed to in vitro GI digestion prior to cellular exposure to simulate digestion conditions in the human body and therefore investigate the role of modulations in the GI tract on the cell response. The effect of digested and undigested hydrolysates on intracellular oxidation, cellular metabolic energy and proteome level was investigated. No difference in the effect on intracellular oxidation activity was obtained between cod and chicken hydrolysates, while higher effect on intracellular oxidation was provided by digested hydrolysates, with relative values of intracellular oxidation of cod of (70.2±0.8) and chicken of (74.5±1.4) % than by undigested ones, where values of cod and chicken were (95.5±1.2) and (90.5±0.7) %, respectively. Neither species nor digestion had any effect on cellular metabolic energy. At proteome level, digested hydrolysates gave again significantly stronger responses than undigested counterparts; cod peptides here also gave somewhat stronger response than chicken peptides. The knowledge of the action of food protein hydrolysates and their digests within live cells, also at proteome level, is important for further validation of their activity in higher eukaryotes to develop new products, such as in this case chicken and cod muscle-derived peptides as functional ingredients. |
format |
Article in Journal/Newspaper |
author |
Jamnik, Polona Istenič, Katja Wulff, Tune Geirsdottir, Margret Almgren, Annette Jonsdottir, Rosa Kristinsson, Hordur Undeland, Ingrid |
author_facet |
Jamnik, Polona Istenič, Katja Wulff, Tune Geirsdottir, Margret Almgren, Annette Jonsdottir, Rosa Kristinsson, Hordur Undeland, Ingrid |
author_sort |
Jamnik, Polona |
title |
Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion |
title_short |
Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion |
title_full |
Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion |
title_fullStr |
Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion |
title_full_unstemmed |
Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion |
title_sort |
bioactivity of cod and chicken protein hydrolysates before and after in vitro gastrointestinal digestion |
publishDate |
2017 |
url |
https://orbit.dtu.dk/en/publications/948e0154-af8b-43f9-9ea2-ef6ab82e6ffa https://doi.org/10.17113/ftb.55.03.17.5117 https://backend.orbit.dtu.dk/ws/files/134357020/Bioactivity_of_Cod_and_Chicken_Protein_Hydrolysates_before_and_after_in_vitro_Gastrointestinal_Digestion.pdf https://backend.orbit.dtu.dk/ws/files/138285503/ftb_55_360.pdf |
genre |
Gadus morhua |
genre_facet |
Gadus morhua |
op_source |
Jamnik , P , Istenič , K , Wulff , T , Geirsdottir , M , Almgren , A , Jonsdottir , R , Kristinsson , H & Undeland , I 2017 , ' Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion ' , Food Technology and Biotechnology , vol. 55 , no. 3 , pp. 360-367 . https://doi.org/10.17113/ftb.55.03.17.5117 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.17113/ftb.55.03.17.5117 |
container_title |
Food Technology and Biotechnology |
container_volume |
55 |
container_issue |
3 |
_version_ |
1766005625797476352 |