Protein phosphorylation profile of Atlantic cod ( Gadus morhua ) in response to pre-slaughter pumping stress and postmortem time
This study aimed to evaluate the effect of pumping stress (pumping and pumping-resting) and postmortem time (before and after rigor mortis ) on phosphorylation profiles of myofibrillar protein (MP) and sarcoplasmic protein (SP) of Atlantic cod ( Gadus morhua ) fillets. The result showed that MP had...
Published in: | Food Chemistry |
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Format: | Article in Journal/Newspaper |
Language: | English |
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2023
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Online Access: | https://orbit.dtu.dk/en/publications/4a71eb96-3e2a-4fec-b931-d7dbe98f8831 https://doi.org/10.1016/j.foodchem.2022.134234 |
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ftdtupubl:oai:pure.atira.dk:publications/4a71eb96-3e2a-4fec-b931-d7dbe98f8831 2024-09-15T17:55:24+00:00 Protein phosphorylation profile of Atlantic cod ( Gadus morhua ) in response to pre-slaughter pumping stress and postmortem time Zhang, Longteng Li, Qian Sørensen, Jonas Steenholdt Luo, Yongkang Lametsch, René 2023 https://orbit.dtu.dk/en/publications/4a71eb96-3e2a-4fec-b931-d7dbe98f8831 https://doi.org/10.1016/j.foodchem.2022.134234 eng eng https://orbit.dtu.dk/en/publications/4a71eb96-3e2a-4fec-b931-d7dbe98f8831 info:eu-repo/semantics/closedAccess Zhang , L , Li , Q , Sørensen , J S , Luo , Y & Lametsch , R 2023 , ' Protein phosphorylation profile of Atlantic cod ( Gadus morhua ) in response to pre-slaughter pumping stress and postmortem time ' , Food Chemistry , vol. 402 , 134234 . https://doi.org/10.1016/j.foodchem.2022.134234 Protein phosphorylation Pumping Postmortem time Myofibrillar proteins Sarcoplasmic proteins article 2023 ftdtupubl https://doi.org/10.1016/j.foodchem.2022.134234 2024-08-13T00:03:06Z This study aimed to evaluate the effect of pumping stress (pumping and pumping-resting) and postmortem time (before and after rigor mortis ) on phosphorylation profiles of myofibrillar protein (MP) and sarcoplasmic protein (SP) of Atlantic cod ( Gadus morhua ) fillets. The result showed that MP had higher global phosphorylation levels than SP regardless of stress condition and postmortem time. The pumping process resulted in significant changes in phosphorylation of structural proteins including myosin heavy and light chains. Pumping also affected the phosphorylation status of heat shock proteins and metabolic enzymes involved in the glycolytic pathways, indicating the possible role of phosphorylation in regulating energy hemostasis of fish under stressful conditions. The pumping-induced phosphorylation changes mainly occurred before rigor mortis , and postmortem time affected the phosphorylation status to a less extent. This work contributes to a deeper understanding on protein phosphorylation affected by pre-slaughter stress and postmortem time of fish. Article in Journal/Newspaper atlantic cod Gadus morhua Technical University of Denmark: DTU Orbit Food Chemistry 402 134234 |
institution |
Open Polar |
collection |
Technical University of Denmark: DTU Orbit |
op_collection_id |
ftdtupubl |
language |
English |
topic |
Protein phosphorylation Pumping Postmortem time Myofibrillar proteins Sarcoplasmic proteins |
spellingShingle |
Protein phosphorylation Pumping Postmortem time Myofibrillar proteins Sarcoplasmic proteins Zhang, Longteng Li, Qian Sørensen, Jonas Steenholdt Luo, Yongkang Lametsch, René Protein phosphorylation profile of Atlantic cod ( Gadus morhua ) in response to pre-slaughter pumping stress and postmortem time |
topic_facet |
Protein phosphorylation Pumping Postmortem time Myofibrillar proteins Sarcoplasmic proteins |
description |
This study aimed to evaluate the effect of pumping stress (pumping and pumping-resting) and postmortem time (before and after rigor mortis ) on phosphorylation profiles of myofibrillar protein (MP) and sarcoplasmic protein (SP) of Atlantic cod ( Gadus morhua ) fillets. The result showed that MP had higher global phosphorylation levels than SP regardless of stress condition and postmortem time. The pumping process resulted in significant changes in phosphorylation of structural proteins including myosin heavy and light chains. Pumping also affected the phosphorylation status of heat shock proteins and metabolic enzymes involved in the glycolytic pathways, indicating the possible role of phosphorylation in regulating energy hemostasis of fish under stressful conditions. The pumping-induced phosphorylation changes mainly occurred before rigor mortis , and postmortem time affected the phosphorylation status to a less extent. This work contributes to a deeper understanding on protein phosphorylation affected by pre-slaughter stress and postmortem time of fish. |
format |
Article in Journal/Newspaper |
author |
Zhang, Longteng Li, Qian Sørensen, Jonas Steenholdt Luo, Yongkang Lametsch, René |
author_facet |
Zhang, Longteng Li, Qian Sørensen, Jonas Steenholdt Luo, Yongkang Lametsch, René |
author_sort |
Zhang, Longteng |
title |
Protein phosphorylation profile of Atlantic cod ( Gadus morhua ) in response to pre-slaughter pumping stress and postmortem time |
title_short |
Protein phosphorylation profile of Atlantic cod ( Gadus morhua ) in response to pre-slaughter pumping stress and postmortem time |
title_full |
Protein phosphorylation profile of Atlantic cod ( Gadus morhua ) in response to pre-slaughter pumping stress and postmortem time |
title_fullStr |
Protein phosphorylation profile of Atlantic cod ( Gadus morhua ) in response to pre-slaughter pumping stress and postmortem time |
title_full_unstemmed |
Protein phosphorylation profile of Atlantic cod ( Gadus morhua ) in response to pre-slaughter pumping stress and postmortem time |
title_sort |
protein phosphorylation profile of atlantic cod ( gadus morhua ) in response to pre-slaughter pumping stress and postmortem time |
publishDate |
2023 |
url |
https://orbit.dtu.dk/en/publications/4a71eb96-3e2a-4fec-b931-d7dbe98f8831 https://doi.org/10.1016/j.foodchem.2022.134234 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
Zhang , L , Li , Q , Sørensen , J S , Luo , Y & Lametsch , R 2023 , ' Protein phosphorylation profile of Atlantic cod ( Gadus morhua ) in response to pre-slaughter pumping stress and postmortem time ' , Food Chemistry , vol. 402 , 134234 . https://doi.org/10.1016/j.foodchem.2022.134234 |
op_relation |
https://orbit.dtu.dk/en/publications/4a71eb96-3e2a-4fec-b931-d7dbe98f8831 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.foodchem.2022.134234 |
container_title |
Food Chemistry |
container_volume |
402 |
container_start_page |
134234 |
_version_ |
1810431684631330816 |