Optimization of reaction parameters for enzymatic glyceride synthesis from fish oil: Ethyl esters versus free fatty acids

• Published in: Biocatalysis and Agricultural Biotechnology
• Main Authors: Ravn, Helle Christine, Damstrup, Marianne L., Meyer, Anne S.
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2012
• Subjects: Immobilized Candida antarctica lipase B; Fish oil concentrates; Omega-3 fatty acids; Central composite design; Esterification; Transesterification; Antarc*; Antarctica
• Online Access: https://orbit.dtu.dk/en/publications/38432f32-dbfa-4f9a-b8f9-bc7393c90aae; https://doi.org/10.1016/j.bcab.2012.06.002

Enzymatic conversion of fish oil free fatty acids (FFA) or fatty acid ethyl esters (FAE) into glycerides via esterification or transesterification was examined. The reactions catalyzed by Lipozyme™ 435, a Candida antarctica lipase, were optimized. Influence on conversion yields of fatty acid chain length, saturation degree, temperature, enzyme dosage, molar ratio glycerol:fatty acids, acyl source composition (w/w ratio FFA:FAE), and reaction time was evaluated collectively by multiple linear regression. All reaction variables influenced the conversion into glycerides. Transesterification of FAE produced the highest yields of 94–95% (w/w) conversion yield at 1:3 glycerol:FAE, 25h, 66°C, enzyme dosage 3.1%w/w.