Optimization of reaction parameters for enzymatic glyceride synthesis from fish oil: Ethyl esters versus free fatty acids

Enzymatic conversion of fish oil free fatty acids (FFA) or fatty acid ethyl esters (FAE) into glycerides via esterification or transesterification was examined. The reactions catalyzed by Lipozyme™ 435, a Candida antarctica lipase, were optimized. Influence on conversion yields of fatty acid chain l...

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Bibliographic Details
Published in:Biocatalysis and Agricultural Biotechnology
Main Authors: Ravn, Helle Christine, Damstrup, Marianne L., Meyer, Anne S.
Format: Article in Journal/Newspaper
Language:English
Published: 2012
Subjects:
Immobilized Candida antarctica lipase B
Fish oil concentrates
Omega-3 fatty acids
Central composite design
Esterification
Transesterification
Antarc*
Antarctica
Online Access:https://orbit.dtu.dk/en/publications/38432f32-dbfa-4f9a-b8f9-bc7393c90aae
https://doi.org/10.1016/j.bcab.2012.06.002
Description
Summary:Enzymatic conversion of fish oil free fatty acids (FFA) or fatty acid ethyl esters (FAE) into glycerides via esterification or transesterification was examined. The reactions catalyzed by Lipozyme™ 435, a Candida antarctica lipase, were optimized. Influence on conversion yields of fatty acid chain length, saturation degree, temperature, enzyme dosage, molar ratio glycerol:fatty acids, acyl source composition (w/w ratio FFA:FAE), and reaction time was evaluated collectively by multiple linear regression. All reaction variables influenced the conversion into glycerides. Transesterification of FAE produced the highest yields of 94–95% (w/w) conversion yield at 1:3 glycerol:FAE, 25h, 66°C, enzyme dosage 3.1%w/w.

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