Dietary methionine level affects growth performance and hepatic gene expression of GH-IGF system and protein turnover regulators in rainbow trout (Oncorhynchus mykiss) fed plant protein-based diets
The effects of dietary level of methionine were investigated in juvenile rainbow trout (Oncorhynchus mykiss) fed five plant-based diets containing increasing content of crystalline methionine (Met), in a six week growth trial. Changes in the hepatic expression of genes related to i) the somatotropic...
Published in: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
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Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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2015
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Online Access: | https://orbit.dtu.dk/en/publications/14b68e63-6a17-4698-8dcf-6c8036688768 https://doi.org/10.1016/j.cbpb.2014.11.009 |
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ftdtupubl:oai:pure.atira.dk:publications/14b68e63-6a17-4698-8dcf-6c8036688768 2024-06-09T07:44:49+00:00 Dietary methionine level affects growth performance and hepatic gene expression of GH-IGF system and protein turnover regulators in rainbow trout (Oncorhynchus mykiss) fed plant protein-based diets Rolland, Marine Dalsgaard, Anne Johanne Tang Holm, Jorgen Gomez-Requeni, Pedro Skov, Peter Vilhelm 2015 https://orbit.dtu.dk/en/publications/14b68e63-6a17-4698-8dcf-6c8036688768 https://doi.org/10.1016/j.cbpb.2014.11.009 eng eng https://orbit.dtu.dk/en/publications/14b68e63-6a17-4698-8dcf-6c8036688768 info:eu-repo/semantics/closedAccess Rolland , M , Dalsgaard , A J T , Holm , J , Gomez-Requeni , P & Skov , P V 2015 , ' Dietary methionine level affects growth performance and hepatic gene expression of GH-IGF system and protein turnover regulators in rainbow trout (Oncorhynchus mykiss) fed plant protein-based diets ' , Comparative Biochemistry and Physiology - Part B: Biochemistry & Molecular Biology , vol. 181 , pp. 33-41 . https://doi.org/10.1016/j.cbpb.2014.11.009 BIOCHEMISTRY ZOOLOGY BREAM SPARUS-AURATA MESSENGER-RNA EXPRESSION FACTOR-BINDING PROTEIN-1 AMINO-ACID-CONCENTRATIONS FACTOR-I DIFFERENTIAL EXPRESSION NITROGEN-UTILIZATION SOMATOTROPIC AXIS ATLANTIC SALMON MUSCLE GROWTH Fish Crystalline amino acid Proteolytic pathways TOR article 2015 ftdtupubl https://doi.org/10.1016/j.cbpb.2014.11.009 2024-05-15T00:00:42Z The effects of dietary level of methionine were investigated in juvenile rainbow trout (Oncorhynchus mykiss) fed five plant-based diets containing increasing content of crystalline methionine (Met), in a six week growth trial. Changes in the hepatic expression of genes related to i) the somatotropic axis: including the growth hormone receptor I (GHR-I), insulin-like growth hormones land II (IGF-I and IGF-II, respectively), and insulin-like growth hormone binding protein-1b (IGFBP-1b); and ii) protein turnover: including the target of rapamycin protein (TOR), proteasome 20 delta (Prot 20D), cathepsin L, calpains 1 and 2 (Capn 1 and Capn 2, respectively), and calpastatin long and short isoforms (CAST-L and CAST-S, respectively) were measured for each dietary treatment. The transcript levels of GHR-I and IGF-I increased linearly with the increase of dietary Met content (P <0.01), reflecting overall growth performances. The apparent capacity for hepatic protein degradation (derived from the gene expression of TOR, Prot 20D, Capn 1, Capn 2, CAST-L and CAST-S) decreased with increasing dietary Met level in a relatively linear manner. Our results suggest that Met availability affects, directly or indirectly, the expression of genes involved in the GH/IGF axis response and protein turnover, which are centrally involved in the regulation of growth. (C) 2014 Elsevier Inc. All rights reserved. Article in Journal/Newspaper Atlantic salmon Technical University of Denmark: DTU Orbit Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 181 33 41 |
institution |
Open Polar |
collection |
Technical University of Denmark: DTU Orbit |
op_collection_id |
ftdtupubl |
language |
English |
topic |
BIOCHEMISTRY ZOOLOGY BREAM SPARUS-AURATA MESSENGER-RNA EXPRESSION FACTOR-BINDING PROTEIN-1 AMINO-ACID-CONCENTRATIONS FACTOR-I DIFFERENTIAL EXPRESSION NITROGEN-UTILIZATION SOMATOTROPIC AXIS ATLANTIC SALMON MUSCLE GROWTH Fish Crystalline amino acid Proteolytic pathways TOR |
spellingShingle |
BIOCHEMISTRY ZOOLOGY BREAM SPARUS-AURATA MESSENGER-RNA EXPRESSION FACTOR-BINDING PROTEIN-1 AMINO-ACID-CONCENTRATIONS FACTOR-I DIFFERENTIAL EXPRESSION NITROGEN-UTILIZATION SOMATOTROPIC AXIS ATLANTIC SALMON MUSCLE GROWTH Fish Crystalline amino acid Proteolytic pathways TOR Rolland, Marine Dalsgaard, Anne Johanne Tang Holm, Jorgen Gomez-Requeni, Pedro Skov, Peter Vilhelm Dietary methionine level affects growth performance and hepatic gene expression of GH-IGF system and protein turnover regulators in rainbow trout (Oncorhynchus mykiss) fed plant protein-based diets |
topic_facet |
BIOCHEMISTRY ZOOLOGY BREAM SPARUS-AURATA MESSENGER-RNA EXPRESSION FACTOR-BINDING PROTEIN-1 AMINO-ACID-CONCENTRATIONS FACTOR-I DIFFERENTIAL EXPRESSION NITROGEN-UTILIZATION SOMATOTROPIC AXIS ATLANTIC SALMON MUSCLE GROWTH Fish Crystalline amino acid Proteolytic pathways TOR |
description |
The effects of dietary level of methionine were investigated in juvenile rainbow trout (Oncorhynchus mykiss) fed five plant-based diets containing increasing content of crystalline methionine (Met), in a six week growth trial. Changes in the hepatic expression of genes related to i) the somatotropic axis: including the growth hormone receptor I (GHR-I), insulin-like growth hormones land II (IGF-I and IGF-II, respectively), and insulin-like growth hormone binding protein-1b (IGFBP-1b); and ii) protein turnover: including the target of rapamycin protein (TOR), proteasome 20 delta (Prot 20D), cathepsin L, calpains 1 and 2 (Capn 1 and Capn 2, respectively), and calpastatin long and short isoforms (CAST-L and CAST-S, respectively) were measured for each dietary treatment. The transcript levels of GHR-I and IGF-I increased linearly with the increase of dietary Met content (P <0.01), reflecting overall growth performances. The apparent capacity for hepatic protein degradation (derived from the gene expression of TOR, Prot 20D, Capn 1, Capn 2, CAST-L and CAST-S) decreased with increasing dietary Met level in a relatively linear manner. Our results suggest that Met availability affects, directly or indirectly, the expression of genes involved in the GH/IGF axis response and protein turnover, which are centrally involved in the regulation of growth. (C) 2014 Elsevier Inc. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Rolland, Marine Dalsgaard, Anne Johanne Tang Holm, Jorgen Gomez-Requeni, Pedro Skov, Peter Vilhelm |
author_facet |
Rolland, Marine Dalsgaard, Anne Johanne Tang Holm, Jorgen Gomez-Requeni, Pedro Skov, Peter Vilhelm |
author_sort |
Rolland, Marine |
title |
Dietary methionine level affects growth performance and hepatic gene expression of GH-IGF system and protein turnover regulators in rainbow trout (Oncorhynchus mykiss) fed plant protein-based diets |
title_short |
Dietary methionine level affects growth performance and hepatic gene expression of GH-IGF system and protein turnover regulators in rainbow trout (Oncorhynchus mykiss) fed plant protein-based diets |
title_full |
Dietary methionine level affects growth performance and hepatic gene expression of GH-IGF system and protein turnover regulators in rainbow trout (Oncorhynchus mykiss) fed plant protein-based diets |
title_fullStr |
Dietary methionine level affects growth performance and hepatic gene expression of GH-IGF system and protein turnover regulators in rainbow trout (Oncorhynchus mykiss) fed plant protein-based diets |
title_full_unstemmed |
Dietary methionine level affects growth performance and hepatic gene expression of GH-IGF system and protein turnover regulators in rainbow trout (Oncorhynchus mykiss) fed plant protein-based diets |
title_sort |
dietary methionine level affects growth performance and hepatic gene expression of gh-igf system and protein turnover regulators in rainbow trout (oncorhynchus mykiss) fed plant protein-based diets |
publishDate |
2015 |
url |
https://orbit.dtu.dk/en/publications/14b68e63-6a17-4698-8dcf-6c8036688768 https://doi.org/10.1016/j.cbpb.2014.11.009 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Rolland , M , Dalsgaard , A J T , Holm , J , Gomez-Requeni , P & Skov , P V 2015 , ' Dietary methionine level affects growth performance and hepatic gene expression of GH-IGF system and protein turnover regulators in rainbow trout (Oncorhynchus mykiss) fed plant protein-based diets ' , Comparative Biochemistry and Physiology - Part B: Biochemistry & Molecular Biology , vol. 181 , pp. 33-41 . https://doi.org/10.1016/j.cbpb.2014.11.009 |
op_relation |
https://orbit.dtu.dk/en/publications/14b68e63-6a17-4698-8dcf-6c8036688768 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.cbpb.2014.11.009 |
container_title |
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
container_volume |
181 |
container_start_page |
33 |
op_container_end_page |
41 |
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1801373645580271616 |