Dietary methionine level affects growth performance and hepatic gene expression of GH-IGF system and protein turnover regulators in rainbow trout (Oncorhynchus mykiss) fed plant protein-based diets

The effects of dietary level of methionine were investigated in juvenile rainbow trout (Oncorhynchus mykiss) fed five plant-based diets containing increasing content of crystalline methionine (Met), in a six week growth trial. Changes in the hepatic expression of genes related to i) the somatotropic...

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Bibliographic Details
Published in:Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
Main Authors: Rolland, Marine, Dalsgaard, Anne Johanne Tang, Holm, Jorgen, Gomez-Requeni, Pedro, Skov, Peter Vilhelm
Format: Article in Journal/Newspaper
Language:English
Published: 2015
Subjects:
BIOCHEMISTRY
ZOOLOGY
BREAM SPARUS-AURATA
MESSENGER-RNA EXPRESSION
FACTOR-BINDING PROTEIN-1
AMINO-ACID-CONCENTRATIONS
FACTOR-I
DIFFERENTIAL EXPRESSION
NITROGEN-UTILIZATION
SOMATOTROPIC AXIS
ATLANTIC SALMON
MUSCLE GROWTH
Fish
Crystalline amino acid
Proteolytic pathways
TOR
Atlantic salmon
Online Access:https://orbit.dtu.dk/en/publications/14b68e63-6a17-4698-8dcf-6c8036688768
https://doi.org/10.1016/j.cbpb.2014.11.009
Description
Summary:The effects of dietary level of methionine were investigated in juvenile rainbow trout (Oncorhynchus mykiss) fed five plant-based diets containing increasing content of crystalline methionine (Met), in a six week growth trial. Changes in the hepatic expression of genes related to i) the somatotropic axis: including the growth hormone receptor I (GHR-I), insulin-like growth hormones land II (IGF-I and IGF-II, respectively), and insulin-like growth hormone binding protein-1b (IGFBP-1b); and ii) protein turnover: including the target of rapamycin protein (TOR), proteasome 20 delta (Prot 20D), cathepsin L, calpains 1 and 2 (Capn 1 and Capn 2, respectively), and calpastatin long and short isoforms (CAST-L and CAST-S, respectively) were measured for each dietary treatment. The transcript levels of GHR-I and IGF-I increased linearly with the increase of dietary Met content (P <0.01), reflecting overall growth performances. The apparent capacity for hepatic protein degradation (derived from the gene expression of TOR, Prot 20D, Capn 1, Capn 2, CAST-L and CAST-S) decreased with increasing dietary Met level in a relatively linear manner. Our results suggest that Met availability affects, directly or indirectly, the expression of genes involved in the GH/IGF axis response and protein turnover, which are centrally involved in the regulation of growth. (C) 2014 Elsevier Inc. All rights reserved.

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