Proof of Concept to Isolate and Culture Primary Muscle Cells from Northern Elephant Seals to Study the Mechanisms that Maintain Aerobic Metabolism Under the Hypoxic Conditions of Breath-hold Diving

LONG-TERM GOALS: To isolate and culture primary muscle cells from the swimming muscles of northern elephant seals. OBJECTIVES: Objective 1. To test the hypothesis that the skeletal muscle cells of diving mammals are preconditioned to function under hypoxic conditions compared to terrestrial models....

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Bibliographic Details
Main Author: Kanatous, Shane B
Other Authors: COLORADO STATE UNIV FORT COLLINS DEPT OF BIOLOGY
Format: Text
Language:English
Published: 2012
Subjects:
Biological Oceanography
*CELLS(BIOLOGY)
*MUSCLES
*SEALS(MAMMALS)
AEROBIC PROCESSES
DIVING
HEMOGLOBIN
MARINE BIOLOGY
METABOLISM
ELEPHANT SEALS
HYPOXIC ENVIRONMENTS
MUSCLE CELLS
Elephant Seals
Online Access:http://www.dtic.mil/docs/citations/ADA573541
http://oai.dtic.mil/oai/oai?&verb=getRecord&metadataPrefix=html&identifier=ADA573541
Description
Summary:LONG-TERM GOALS: To isolate and culture primary muscle cells from the swimming muscles of northern elephant seals. OBJECTIVES: Objective 1. To test the hypothesis that the skeletal muscle cells of diving mammals are preconditioned to function under hypoxic conditions compared to terrestrial models. Specifically, proteins and transcripts associated with increased oxygen storage, lipid catabolism and hypoxic pathways will be upregulated in seal primary cells as compared to terrestrial models. Objective 2. To test the hypothesis, that varying levels of stimulation and/or hypoxic conditions will differentially regulate the expression of myoglobin. The original document contains color images.

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