Immunodiagnostic Approaches to Diseases in Marine Mammals

The rapid diagnosis of infectious diseases in all animals has been the major focus of clinical microbiology for decades. It is particularly important in marine mammals since illness, with cessation of eating, causes rapid dehydration with serious sequelae. Initial studies indicated that commercial a...

Full description

Bibliographic Details
Main Authors: Vedros, Neylan A., Schroeder, J. P., Suer, L.
Other Authors: NAVAL OCEAN SYSTEMS CENTER SAN DIEGO CA
Format: Text
Language:English
Published: 1987
Subjects:
Biochemistry
Medicine and Medical Research
*INFECTIOUS DISEASES
*DIAGNOSIS(MEDICINE)
*IMMUNOLOGY
QUICK REACTION
MAMMALS
POLYSACCHARIDES
ENZYMES
DISEASES
SURFACES
BASE LINES
DIAGNOSIS(GENERAL)
ANTIBODIES
ANIMALS
DIFFUSION
NORTH(DIRECTION)
IMMUNE SERUMS
MONOCLONAL ANTIBODIES
IMMUNITY
IMMUNOASSAY
SEROLOGY
ASSAYING
ANTIGENS
SEALS(STOPPERS)
DEHYDRATION
RABBITS
AQUATIC ANIMALS
SEALS(MAMMALS)
CETACEA
GUINEA PIGS
DOLPHINS(MAMMALS)
PORPOISES
BLASTOMYCES
CANDIDA
COCCIDIOIDES
FUR
REPRINTS
HISTOPLASMA
TEST AND EVALUATION
CONTROL
Hilton
Callorhinus ursinus
Northern fur seal
Online Access:http://www.dtic.mil/docs/citations/ADA216659
http://oai.dtic.mil/oai/oai?&verb=getRecord&metadataPrefix=html&identifier=ADA216659
Description
Summary:The rapid diagnosis of infectious diseases in all animals has been the major focus of clinical microbiology for decades. It is particularly important in marine mammals since illness, with cessation of eating, causes rapid dehydration with serious sequelae. Initial studies indicated that commercial anti-Ig's of a variety of animals were not suitable for use in serological studies with marine mammals. The IgG and IgM of cetaceans and pinnipeds were isolated, antisera produced in rabbits and guinea pigs, and used to develop the single radial immunodiffusion (RID) immunoblot, and ELISA techniques. The base-line level of IgG and IgM in the bottlenose dolphin (Tursiops truncatus), the sea otter (Enhydra lutris), and the northern fur seal (Callorhinus ursinus) have been determined by RID. Specific antigens and monoclonal antibodies as positive controls for Histoplasma, Blastomyces, Coccidioides, Candida, and Aspergillus have been developed. Specific outermembrane proteins of Erysipelothrix rhusiopathiae and surface polysaccharides of Pasturella multocida and Pseudomonas pseudomallei have been used successfully in an ELISA assay. The potential of all of the above diagnostic tests for infectious diseases in marine mammals will be discussed. Reprints. Published in the Proceedings of the International Conference on Zoological and Avian Medicine (1st) held at the Turtle Bay Hilton and Country Club, Oahu, HI on 6-11 Sep 1987.

Similar Items