Filtered sequencing data (MOTUs) for fungal metabarcode

This table contains filtered MOTUs for the fungal metabarcode. Amplicons were amplified using ITS5 : 5'-GGAAGTAAAAGTCGTAACAAGG-3' (White et al. 1990) and 5.8S_fungi : 5'- CAAGAGATCCGTTGTTGAAAGTT-3' primers (Epp et al. 2012). Sequences were obtained by a 2 x 250 bp paired-end sequ...

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Bibliographic Details
Main Authors: Pansu, Johan, Winkworth, Richard C., Hennion, Françoise, Gielly, Ludovic, Taberlet, Pierre, Choler, Philippe
Format: Report
Language:unknown
Published: 2015
Subjects:
Environmental DNA
Invasive species
Metabarcoding
Soil communities
Antarctic
Kerguelen
Kerguelen Islands
Antarc*
Online Access:http://hdl.handle.net/10255/dryad.93607
https://doi.org/10.5061/dryad.t8534/4
Description
Summary:This table contains filtered MOTUs for the fungal metabarcode. Amplicons were amplified using ITS5 : 5'-GGAAGTAAAAGTCGTAACAAGG-3' (White et al. 1990) and 5.8S_fungi : 5'- CAAGAGATCCGTTGTTGAAAGTT-3' primers (Epp et al. 2012). Sequences were obtained by a 2 x 250 bp paired-end sequencing on Illumina MiSeq platform. Data filtering and MOTUs clustering were performed according to the procedure described in the supplementary materials of Pansu et al. (2015): 'Long-lasting modification of soil fungal diversity associated with the introduction of rabbits to a remote sub-Antarctic archipelago'.

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