Microsatellite Primers

Sputnik software was used to search di-, tri-, tetra-, pena- and hex-nucleotide motif SSRs. Primers were designed using the Primer3 package.After aligning the amplicon sequences to the Atlantic salmon genome with BLAT, primers were chose because their amplicons were located in the genome of Atlantic...

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Bibliographic Details
Main Authors: Tong, Guang-Xiang, Xu, Wei, Zhang, Yong-Quan, Zhang, Qing-Yu, Yin, Jia-Sheng, Kuang, Youyi
Format: Dataset
Language:unknown
Published: 2017
Subjects:
RNA-Seq
comparative transcript analysis
positive selection
microsatellite markers
Pena
Sputnik
Atlantic salmon
Online Access:http://hdl.handle.net/10255/dryad.152478
https://doi.org/10.5061/dryad.9gd3n/9
Description
Summary:Sputnik software was used to search di-, tri-, tetra-, pena- and hex-nucleotide motif SSRs. Primers were designed using the Primer3 package.After aligning the amplicon sequences to the Atlantic salmon genome with BLAT, primers were chose because their amplicons were located in the genome of Atlantic salmon with identities >70% and spanned distances close to the length of the amplicons.

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