Microsatellite Primers
Sputnik software was used to search di-, tri-, tetra-, pena- and hex-nucleotide motif SSRs. Primers were designed using the Primer3 package.After aligning the amplicon sequences to the Atlantic salmon genome with BLAT, primers were chose because their amplicons were located in the genome of Atlantic...
Main Authors: | , , , , , |
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Format: | Dataset |
Language: | unknown |
Published: |
2017
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Subjects: | |
Online Access: | http://hdl.handle.net/10255/dryad.152478 https://doi.org/10.5061/dryad.9gd3n/9 |
Summary: | Sputnik software was used to search di-, tri-, tetra-, pena- and hex-nucleotide motif SSRs. Primers were designed using the Primer3 package.After aligning the amplicon sequences to the Atlantic salmon genome with BLAT, primers were chose because their amplicons were located in the genome of Atlantic salmon with identities >70% and spanned distances close to the length of the amplicons. |
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