Summary: | Kirstine Nolling Jensen,1,2 Marieke Heijink,3 Martin Giera,3 Jona Freysdottir,1,2,* Ingibjorg Hardardottir1,2,* 1Faculty of Medicine, Biomedical Center, University of Iceland, Reykjavik, Iceland; 2Department of Immunology, Landspitali – The National University Hospital of Iceland, Reykjavik, Iceland; 3Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, the Netherlands*These authors contributed equally to this workCorrespondence: Ingibjorg Hardardottir Tel +354 525 4885Email ih@hi.isPurpose: To determine the effects of dietary omega-3 polyunsaturated fatty acids (PUFAs) on recruitment of natural killer (NK) cells and resolution responses in antigen-induced peritonitis in mice.Methods: Mice were fed fish oil-enriched or control diets, immunized twice and challenged intraperitoneally with methylated bovine serum albumin. Prior to and at different time-points following inflammation induction, expression of surface molecules on peritoneal cells was determined by flow cytometry, concentration of soluble mediators in peritoneal fluid by ELISA or Luminex, and of lipid mediators by LC-MS/MS, and number of apoptotic cells in mesenteric lymph nodes by TUNEL staining.Results: Mice fed the fish oil diet had higher number of CD11b+CD27− NK cells as well as a higher proportion of CD107a+ NK cells in their peritoneum 6 h after inflammation induction than mice fed the control diet. They also had higher numbers of CCR5+ NK cells and higher concentrations of CCL5 and CXCL12. Additionally, a higher fraction of apoptotic neutrophils but lower fraction of CD47+ neutrophils were present in the peritoneum of mice fed the fish oil diet 6 h after inflammation induction and the fish oil fed mice had a shorter resolution interval. They also had lower concentrations of pro-inflammatory mediators but higher concentrations of the anti-inflammatory/pro-resolution mediators TGF-β, IGF-1, and soluble TNF RII, as well as higher ratios of hydroxyeicosapentaenoic acid (HEPE) to hydroxyeicosatetraenoic acid (HETE) ...
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