MOLECULAR INVESTIGATION OF HEMOTROPIC MYCOPLASMAS IN HUMAN BEINGS, DOGS AND HORSES IN A RURAL SETTLEMENT IN SOUTHERN BRAZIL

SUMMARY The aims of this study were to determine the prevalence of hemoplasmas in a rural Brazilian settlement's population of human beings, their dogs and horses, highly exposed to tick bites; to identify the tick species parasitizing dogs and horses, and analyze factors associated with their...

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Bibliographic Details
Published in:Revista do Instituto de Medicina Tropical de São Paulo
Main Authors: Rafael Felipe da Costa VIEIRA, Odilon VIDOTTO, Thállitha Samih Wischral Jayme VIEIRA, Ana Márcia Sá GUIMARAES, Andrea Pires dos SANTOS, Naíla Cannes NASCIMENTO, Nelson Jesse Rodrigues dos SANTOS, Thiago Fernandes MARTINS, Marcelo Bahia LABRUNA, Mary MARCONDES, Alexander Welker BIONDO, Joanne Belle MESSICK
Format: Article in Journal/Newspaper
Language:English
Published: Universidade de São Paulo (USP) 2015
Subjects:
Hemoplasma
Mycoplasma haemocanis
' CandidatusMycoplasma haematoparvum'
Real-time PCR
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Arctic
Online Access:https://doi.org/10.1590/S0036-46652015000400014
https://doaj.org/article/fe9fd1e79a8e49698ddcdb6ce93a0e7b
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Summary:SUMMARY The aims of this study were to determine the prevalence of hemoplasmas in a rural Brazilian settlement's population of human beings, their dogs and horses, highly exposed to tick bites; to identify the tick species parasitizing dogs and horses, and analyze factors associated with their infection. Blood samples from 132 dogs, 16 horses and 100 humans were screened using a pan-hemoplasma SYBR green real-time PCR assay followed by a species-specific TaqMan real-time PCR. A total of 59/132 (44.7%) dog samples were positive for hemoplasmas (21 Mycoplasma haemocanisalone, 12 ' Candidatus Mycoplasma haematoparvum' alone and 21 both). Only 1/100 (1.0%) human sample was positive by qPCR SYBR green, with no successful amplification of 16S rRNA or 23 rRNA genes despite multiple attempts. All horse samples were negative. Dogs >1 year of age were more likely to be positive for hemoplasmas ( p= 0.0014). In conclusion, although canine hemoplasma infection was highly prevalent, cross-species hemoplasma transmission was not observed, and therefore may not frequently occur despite overexposure of agents and vectors.

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