Evaluation of miRNA-143 and miRNA-145 Expression and Their Association with Vitamin-D Status Among Obese and Non-Obese Type-2 Diabetic Patients

• Main Authors: Aladel A, Khatoon F, Khan MI, Alsheweir A, Almutairi MG, Almutairi SO, Almutairi FK, Osmonaliev K, Beg MMA
• Format: Article in Journal/Newspaper
• Language: English
• Published: Dove Medical Press 2022
• Subjects: type 2 diabetes; obesity; vitamin-d; mirna; expression; prognostic marker; Medicine (General); R5-920; sami
• Online Access: https://doaj.org/article/fcbc38f237164f8e9c07eca05d9ec109

Alanoud Aladel,1 Fahmida Khatoon,2 Mohammad Idreesh Khan,3 Azzah Alsheweir,1 Malak Ghazi Almutairi,4 Sami Owaidh Almutairi,4 Faisal Khalid Almutairi,5 Kudaibergen Osmonaliev,6 Mirza Masroor Ali Beg6,7 1Department of Community Health Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia; 2Biochemistry Department, College of Medicine, University of Ha’il, Ha’il, Saudi Arabia; 3Department of Clinical Nutrition, College of Applied Health Sciences in Ar Rass, Qassim University, Ar Rass, 51921, Saudi Arabia; 4Department of Clinical Nutrition, Almethnab General Hospital, Qassim Health Cluster, Ministry of Health, Al Mithnab, Saudi Arabia; 5Laboratory Department, Armed Forces Hospital in Qassim, Medical Services, Ministry of Defense Qassim Buraydah Al-Rass, Buraydah, Saudi Arabia; 6Faculty of Medicine, Alatoo International University, Bishkek, Kyrgyzstan; 7Centre for Promotion of Medical Research, Bishkek, KyrgyzstanCorrespondence: Mirza Masroor Ali Beg, Email [e-mail]: Growing epidemics of type-2 diabetes mellitus (T2DM) and obesity have become a serious health concern. Since miRNAs and vitamin levels affect the development and progression of numerous pathogenic diseases, including diabetes, the present study aimed to evaluate miRNA-143 and miRNA-145 expression and vitamin-D status among obese and non-obese T2DM patients.Methods: The study included 100 clinically confirmed newly diagnosed obese and non-obese T2DM cases and 100 healthy subjects. Total RNA was extracted from collected blood samples and 100 ng of RNA was used for cDNA synthesis, then TaqMan assay was performed to evaluate the miRNA-143 and miRNA-145 relative expression.Results: T2DM cases with hypertension (4.08 fold, p=0.01; 5.36 fold, p=0.009), fatigue (5.07 fold, p=0.04; 5.32 fold, p=0.03) and blurred vision (5.15 fold, p=0.01) showed higher miRNA-143 and miRNA-145 relative expression compared with their counterparts, respectively. A positive correlation was observed between miRNA-143 and ...