Computed Tomography as a Method for Age Determination of Carnivora and Odontocetes with Validation from Individuals with Known Age

Traditional methods for age determination of wildlife include either slicing thin sections off or grinding a tooth, both of which are laborious and invasive. Especially when it comes to ancient and valuable museum samples of rare or extinct species, non-invasive methods are preferable. In this study...

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Bibliographic Details
Published in:Animals
Main Authors: Sina Baier-Stegmaier, Carsten Gundlach, Mariann Chriél, Mette Sif Hansen, Christina Vedel-Smith, Charlotte Vikkelsø Hansen, Daniel Klingberg Johansson, Louise Birgitte Henriksen, Magnus Wahlberg, Charlotte Bie Thøstesen, Aage Kristian Olsen Alstrup, Kristian Murphy Gregersen, Cino Pertoldi, Sussie Pagh
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2023
Subjects:
museum collections
X-ray
computed tomography
micro-CT
µ-CT
toothed whale age
Veterinary medicine
SF600-1100
Zoology
QL1-991
Phocoena phocoena
toothed whale
Online Access:https://doi.org/10.3390/ani13111783
https://doaj.org/article/f94f72df73de4f98a20b53bf9ce73a8d
Description
Summary:Traditional methods for age determination of wildlife include either slicing thin sections off or grinding a tooth, both of which are laborious and invasive. Especially when it comes to ancient and valuable museum samples of rare or extinct species, non-invasive methods are preferable. In this study, X-ray micro-computed tomography (µ-CT) was verified as an alternative non-invasive method for age determination of three species within the order of Carnivora and suborders Odontoceti. Teeth from 13 red foxes ( Vulpes vulpes ), 2 American mink ( Neogale vison ), and 2 harbor porpoises ( Phocoena phocoena ) of known age were studied using µ-CT. The number of visible dental growth layers in the µ-CT were highly correlated with true age for all three species (R 2 = 96%, p < 0.001). In addition, the Bland–Altman plot showed high agreement between the age of individuals and visible dental layers represented in 2D slices of the 3D µ-CT images. The true age of individuals was on average 0.3 (±0.6 SD) years higher than the age interpreted by the µ-CT image, and there was a 95% agreement between the true age and the age interpreted from visible dental layers in the µ-CT.

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