Effective Aeromonas specific monoclonal antibody for immunodiagnosis
Objective: To identify the monoclonal antibody specific to Aeromonas spp., a Gram negative bacteria causing gastroenteritis and wound infection. Methods: The monoclone, namely 88F2-3F4, was produced from hybridoma technology. The specificity of antibody secreted from 88F2-3F4 was tested against othe...
| Published in: | Asian Pacific Journal of Tropical Biomedicine |
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| Main Authors: | , , , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2018
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| Subjects: | |
| Online Access: | https://doi.org/10.4103/2221-1691.221128 https://doaj.org/article/f7b1be087abf43369c22ae79a7eedc3a |
| Summary: | Objective: To identify the monoclonal antibody specific to Aeromonas spp., a Gram negative bacteria causing gastroenteritis and wound infection. Methods: The monoclone, namely 88F2-3F4, was produced from hybridoma technology. The specificity of antibody secreted from 88F2-3F4 was tested against other Gram negative bacteria frequently found in gastrointestinal tract. Then the antibody was used for searching Aeromonas antigens in artificial seeded rectal swab cultures by dot-blot enzyme linked immunosorbent assay. Results: 88F2-3F4 produced an antibody that recognized an antigen with a molecular mass of 8.5 kDa in all 123 isolates of the seven Aeromonas species tested, but recognized no epitope of any other Gram-negative bacterium typically found in the gastrointestinal tract. A dot-blot enzyme linked immunosorbent assay based on this antibody showed 86.49% sensitivity and 92.13% specificity. Conclusions: 88F2-3F4 monoclonal antibody could react with all Aeromonas isolates, but not other Gram negative bacteria, therefore it should be a useful tool for the detection of Aeromonas antigen in clinical and environmental samples. |
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