Identification and Isolation Pattern of Globisporangium spp. from a Sanionia Moss Colony in Ny-Ålesund, Spitsbergen Is., Norway from 2006 to 2018

Globisporangium spp. are soil-inhabiting oomycetes distributed worldwide, including in polar regions. Some species of the genus are known as important plant pathogens. This study aimed to clarify the species construction of Globisporangium spp. and their long-term isolation pattern in Sanionia moss...

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Bibliographic Details
Published in:Microorganisms
Main Authors: Motoaki Tojo, Natsumi Fujii, Hironori Yagi, Yuki Yamashita, Katsuyuki Tokura, Kenichi Kida, Akiho Hakoda, María-Luz Herrero, Tamotsu Hoshino, Masaki Uchida
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2021
Subjects:
Arctic region
long-term population changes
Globisporangium
Pythium
moss
plant pathogens
Biology (General)
QH301-705.5
Arctic
Norway
Ny-Ålesund
Ny Ålesund
Spitsbergen
Online Access:https://doi.org/10.3390/microorganisms9091912
https://doaj.org/article/f419d933dfe142428b6c4c21fb4e90b8
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Summary:Globisporangium spp. are soil-inhabiting oomycetes distributed worldwide, including in polar regions. Some species of the genus are known as important plant pathogens. This study aimed to clarify the species construction of Globisporangium spp. and their long-term isolation pattern in Sanionia moss in Ny-Ålesund, Spitsbergen Is., Norway. Globisporangium spp. were isolated at two-year intervals between 2006 and 2018 at a Sanionia moss colony, Ny-Ålesund, Spitsbergen Is., Norway. The isolates were obtained by using three agar media and were identified based on sequences of the rDNA-ITS region and cultural characteristics. Most of the Globisporangium isolates obtained during the survey were identified into six species. All six species were grown at 0 °C on an agar plate and used to infect Sanionia moss at 4 and/or 10 °C under an in vitro inoculation test. The total isolation frequency of Globisporangium gradually decreased throughout the survey period. The isolation frequency varied among the six species, and four of the species that showed a high frequency in 2006 were rarely isolated after 2016. The results suggested that Globisporangium inhabiting Sanionia moss in Ny-Ålesund has a unique composition of species and that most of the species reduced their population over the recent decade.

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