Immunodiagnosis of hydatid disease: evaluation of antigens from hydatid cyst fluid and the vesicularfluid of Taenia crassiceps metacestode Inmunodiagnóstico de hidatidosis: evaluación de líquido hidático y de líquido vesicular de cisticerco de Taenia crassiceps
The specificity and sensitivity of the enzyme immunoassay (EIA), presently used in South America areas where hydatidosis caused by Echinococcus granulosus is endemic, was compared to two alternative EIA. One of these uses an hydatid antigen of different prepraration and the other vesicular fluid of...
| Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
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| Main Authors: | , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Universidade de São Paulo (USP)
1993
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| Subjects: | |
| Online Access: | https://doi.org/10.1590/S0036-46651993000200007 https://doaj.org/article/f3aa8f679d0d4a6e999c7c33ef2891c4 |
| Summary: | The specificity and sensitivity of the enzyme immunoassay (EIA), presently used in South America areas where hydatidosis caused by Echinococcus granulosus is endemic, was compared to two alternative EIA. One of these uses an hydatid antigen of different prepraration and the other vesicular fluid of Taenia crassiceps cisticerci (VFCC). The effect of previous neutralization in the serum sample of antibodies anti-normal ovine or murine sera and anti-phosphorylcholine on the diagnostic efficiency of these EIA was studied. The frequency of distribution of the titers obtained with normal sera, hydatid sera positive to DD5 test and hydatid sera negative to DD5 test in three EIA systems was analyzed. Results showed a significant decrease of sensitivity of the EIA using VFCC when compared to these EIA using hydatid antigens. This makes inconvenient the use of VFCC for the immunodiagnosis of hydatid disease. No significant differences between the two EIA using hydatid antigens were observed. SDS-PAGE analysis showed remarkable differences between the VFCC and the hydatid antigens composition and some differences among these latters probably due to manufacturing procedures. Se comparó la especificidad y sensibilidad del ensayo inmunoenzimático (EIE) actualmente en uso en las áreas de Sudamérica donde la hidatidosis causada por Echinococcus granulosus es endémica, con dos versiones de EIE, una de las cuales emplea un antígeno hidatídico de diferente preparación y la otra líquido vesicular de cisticerco de Taenia crassiceps (LVCC). Se estudió también para ambos antígenos el efecto de la neutralización previa de los anticuerpos anticomponentes séricos ovinos o murinos, y antifosforilcolina sobre la eficiencia diagnóstica de los EIE. Se analizó la frecuencia de distribución de los títulos obtenidos en los tres sistemas de EIE frente a sueros normales, hidatídicos a la prueba de doble difusión arco 5 (DD5) e hidatídicos DD5 negativos. Los resultados mostraron una significativa disminución de sensibilidad del LVCC frente a los ... |
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