Development and Characterization of 10 Microsatellite Markers in Sagina nodosa (Caryophyllaceae)

• Published in: Applications in Plant Sciences
• Main Authors: Tarjinder Singh, Joan Edwards, Luana S. Maroja
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2014
• Subjects: 454 sequencing; arctic-alpine plant; knotted pearlwort; polymorphism; population genetics; Sagina nodosa; Biology (General); QH301-705.5; Botany; QK1-989; Arctic
• Online Access: https://doi.org/10.3732/apps.1300064; https://doaj.org/article/f2c7b7b99d2040319b11effa7f0eca39

Premise of the study: We developed 10 novel microsatellite loci for Sagina nodosa , a diploid perennial arctic-alpine herb. To our knowledge, these are the first microsatellite loci for a Sagina species. Methods and Results: We performed a low-coverage 454 next-generation sequencing of enriched genomic fragments derived from one individual to generate a massive library of contigs containing potential polymorphic microsatellites. We present data for 10 novel polymorphic microsatellite loci containing di-, tri-, tetra-, and hexanucleotide repeats with two to nine alleles per locus assessed in 29 individuals. Conclusions: These polymorphic microsatellite loci in S. nodosa will provide insights on the population structure and life history of S. nodosa in Isle Royale and other North American populations.