Development and Characterization of 10 Microsatellite Markers in Sagina nodosa (Caryophyllaceae)
Premise of the study: We developed 10 novel microsatellite loci for Sagina nodosa , a diploid perennial arctic-alpine herb. To our knowledge, these are the first microsatellite loci for a Sagina species. Methods and Results: We performed a low-coverage 454 next-generation sequencing of enriched geno...
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ftdoajarticles:oai:doaj.org/article:f2c7b7b99d2040319b11effa7f0eca39 2023-05-15T14:53:29+02:00 Development and Characterization of 10 Microsatellite Markers in Sagina nodosa (Caryophyllaceae) Tarjinder Singh Joan Edwards Luana S. Maroja 2014-01-01T00:00:00Z https://doi.org/10.3732/apps.1300064 https://doaj.org/article/f2c7b7b99d2040319b11effa7f0eca39 EN eng Wiley http://www.bioone.org/doi/full/10.3732/apps.1300064 https://doaj.org/toc/2168-0450 doi:10.3732/apps.1300064 2168-0450 https://doaj.org/article/f2c7b7b99d2040319b11effa7f0eca39 Applications in Plant Sciences, Vol 2, Iss 1, p 1300064 (2014) 454 sequencing arctic-alpine plant knotted pearlwort polymorphism population genetics Sagina nodosa Biology (General) QH301-705.5 Botany QK1-989 article 2014 ftdoajarticles https://doi.org/10.3732/apps.1300064 2022-12-31T08:58:19Z Premise of the study: We developed 10 novel microsatellite loci for Sagina nodosa , a diploid perennial arctic-alpine herb. To our knowledge, these are the first microsatellite loci for a Sagina species. Methods and Results: We performed a low-coverage 454 next-generation sequencing of enriched genomic fragments derived from one individual to generate a massive library of contigs containing potential polymorphic microsatellites. We present data for 10 novel polymorphic microsatellite loci containing di-, tri-, tetra-, and hexanucleotide repeats with two to nine alleles per locus assessed in 29 individuals. Conclusions: These polymorphic microsatellite loci in S. nodosa will provide insights on the population structure and life history of S. nodosa in Isle Royale and other North American populations. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Applications in Plant Sciences 2 1 1300064 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
454 sequencing arctic-alpine plant knotted pearlwort polymorphism population genetics Sagina nodosa Biology (General) QH301-705.5 Botany QK1-989 |
spellingShingle |
454 sequencing arctic-alpine plant knotted pearlwort polymorphism population genetics Sagina nodosa Biology (General) QH301-705.5 Botany QK1-989 Tarjinder Singh Joan Edwards Luana S. Maroja Development and Characterization of 10 Microsatellite Markers in Sagina nodosa (Caryophyllaceae) |
topic_facet |
454 sequencing arctic-alpine plant knotted pearlwort polymorphism population genetics Sagina nodosa Biology (General) QH301-705.5 Botany QK1-989 |
description |
Premise of the study: We developed 10 novel microsatellite loci for Sagina nodosa , a diploid perennial arctic-alpine herb. To our knowledge, these are the first microsatellite loci for a Sagina species. Methods and Results: We performed a low-coverage 454 next-generation sequencing of enriched genomic fragments derived from one individual to generate a massive library of contigs containing potential polymorphic microsatellites. We present data for 10 novel polymorphic microsatellite loci containing di-, tri-, tetra-, and hexanucleotide repeats with two to nine alleles per locus assessed in 29 individuals. Conclusions: These polymorphic microsatellite loci in S. nodosa will provide insights on the population structure and life history of S. nodosa in Isle Royale and other North American populations. |
format |
Article in Journal/Newspaper |
author |
Tarjinder Singh Joan Edwards Luana S. Maroja |
author_facet |
Tarjinder Singh Joan Edwards Luana S. Maroja |
author_sort |
Tarjinder Singh |
title |
Development and Characterization of 10 Microsatellite Markers in Sagina nodosa (Caryophyllaceae) |
title_short |
Development and Characterization of 10 Microsatellite Markers in Sagina nodosa (Caryophyllaceae) |
title_full |
Development and Characterization of 10 Microsatellite Markers in Sagina nodosa (Caryophyllaceae) |
title_fullStr |
Development and Characterization of 10 Microsatellite Markers in Sagina nodosa (Caryophyllaceae) |
title_full_unstemmed |
Development and Characterization of 10 Microsatellite Markers in Sagina nodosa (Caryophyllaceae) |
title_sort |
development and characterization of 10 microsatellite markers in sagina nodosa (caryophyllaceae) |
publisher |
Wiley |
publishDate |
2014 |
url |
https://doi.org/10.3732/apps.1300064 https://doaj.org/article/f2c7b7b99d2040319b11effa7f0eca39 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Applications in Plant Sciences, Vol 2, Iss 1, p 1300064 (2014) |
op_relation |
http://www.bioone.org/doi/full/10.3732/apps.1300064 https://doaj.org/toc/2168-0450 doi:10.3732/apps.1300064 2168-0450 https://doaj.org/article/f2c7b7b99d2040319b11effa7f0eca39 |
op_doi |
https://doi.org/10.3732/apps.1300064 |
container_title |
Applications in Plant Sciences |
container_volume |
2 |
container_issue |
1 |
container_start_page |
1300064 |
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1766325069516111872 |