Development and Characterization of 10 Microsatellite Markers in Sagina nodosa (Caryophyllaceae)

Premise of the study: We developed 10 novel microsatellite loci for Sagina nodosa , a diploid perennial arctic-alpine herb. To our knowledge, these are the first microsatellite loci for a Sagina species. Methods and Results: We performed a low-coverage 454 next-generation sequencing of enriched geno...

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Bibliographic Details
Published in:Applications in Plant Sciences
Main Authors: Tarjinder Singh, Joan Edwards, Luana S. Maroja
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2014
Subjects:
454 sequencing
arctic-alpine plant
knotted pearlwort
polymorphism
population genetics
Sagina nodosa
Biology (General)
QH301-705.5
Botany
QK1-989
Arctic
Online Access:https://doi.org/10.3732/apps.1300064
https://doaj.org/article/f2c7b7b99d2040319b11effa7f0eca39
Description
Summary:Premise of the study: We developed 10 novel microsatellite loci for Sagina nodosa , a diploid perennial arctic-alpine herb. To our knowledge, these are the first microsatellite loci for a Sagina species. Methods and Results: We performed a low-coverage 454 next-generation sequencing of enriched genomic fragments derived from one individual to generate a massive library of contigs containing potential polymorphic microsatellites. We present data for 10 novel polymorphic microsatellite loci containing di-, tri-, tetra-, and hexanucleotide repeats with two to nine alleles per locus assessed in 29 individuals. Conclusions: These polymorphic microsatellite loci in S. nodosa will provide insights on the population structure and life history of S. nodosa in Isle Royale and other North American populations.

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