Low sensitivity of polymerase chain reaction for diagnosis of tuberculous meningitis in southeastern Brazil

Two polymerase chain reaction (PCR) protocols showed low sensitivity (36% and 53% for TB AMPLICOR and MPB64 nested PCR, respectively), when compared with classic microbiological methods (73% and 54% for Ziehl-Neelsen staining and culture, respectively), in the diagnosis of tuberculous meningitis in...

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Bibliographic Details
Main Authors: Vânia Maria Sabadoto Brienze, Ângela Pedroso Tonon, Fabrício José Tarelho Pereira, Elisabete Liso, Waldir Antônio Tognola, Manoel Armando Azevedo dos Santos, Marcelo Urbano Ferreira
Format: Article in Journal/Newspaper
Language:English
Published: Sociedade Brasileira de Medicina Tropical (SBMT) 2001
Subjects:
Diagnosis of tuberculous meningitis
PCR
Mycobacterium tuberculosis
Arctic medicine. Tropical medicine
RC955-962
Arctic
Online Access:https://doaj.org/article/ef5b991d200f4459b4de754e93c52414
Description
Summary:Two polymerase chain reaction (PCR) protocols showed low sensitivity (36% and 53% for TB AMPLICOR and MPB64 nested PCR, respectively), when compared with classic microbiological methods (73% and 54% for Ziehl-Neelsen staining and culture, respectively), in the diagnosis of tuberculous meningitis in 91 patients in southeastern Brazil. Only three PCR-positive, microbiologically negative patients were found. Analysis of sequential cerebrospinal fluid samples by nested PCR detected Mycobacterium tuberculosis DNA up to 29 days after the introduction of antituberculosis chemotherapy.

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