Effect of Marine-Derived Ice-Binding Proteins on the Cryopreservation of Marine Microalgae

Ice-binding protein (IBPs) protect cells from cryo-injury during cryopreservation by inhibiting ice recrystallization (IR), which is a main cause of cell death. In the present study, we employed two IBPs, one, designated LeIBP from Arctic yeast, and the other, designated FfIBP from Antarctic sea ice...

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Bibliographic Details
Published in:Marine Drugs
Main Authors: Hak Jun Kim, Bon-Won Koo, Doa Kim, Ye Seul Seo, Yoon Kwon Nam
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2017
Subjects:
ice-binding proteins
ice recrystallization inhibition
cryoprotectant
slow-freezing
Isochrysis galbana
Pavlova viridis
Chlamydomonas coccoides
Biology (General)
QH301-705.5
Arctic
Antarctic
Pavlova
Antarc*
Sea ice
Online Access:https://doi.org/10.3390/md15120372
https://doaj.org/article/ee2d090abb364873be62704ea505a966
Description
Summary:Ice-binding protein (IBPs) protect cells from cryo-injury during cryopreservation by inhibiting ice recrystallization (IR), which is a main cause of cell death. In the present study, we employed two IBPs, one, designated LeIBP from Arctic yeast, and the other, designated FfIBP from Antarctic sea ice bacterium, in the cryopreservation of three economically valuable marine microalgae, Isochrysis galbana, Pavlova viridis, and Chlamydomonas coccoides. Both of the IBPs showed IR inhibition in f/2 medium containing 10% DMSO, indicating that they retain their function in freezing media. Microalgal cells were frozen in 10% DMSO with or without IBP. Post-thaw viability exhibited that the supplementation of IBPs increased the viability of all cryopreserved cells. LeIBP was effective in P. viridis and C. coccoides, while FfIBP was in I. galbana. The cryopreservative effect was more drastic with P. viridis when 0.05 mg/mL LeIBP was used. These results clearly demonstrate that IBPs could improve the viability of cryopreserved microalgal cells.

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