Transmission competence of a new mesonivirus, Yichang virus, in mosquitoes and its interference with representative flaviviruses.
Advances in technology have greatly stimulated the understanding of insect-specific viruses (ISVs). Unfortunately, most of these findings are based on sequencing technology, and laboratory data are scarce on the transmission dynamics of ISVs in nature and the potential effects of these viruses on ar...
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ftdoajarticles:oai:doaj.org/article:ec011a1da26b41ad80e9996410f0eb35 2023-05-15T15:10:07+02:00 Transmission competence of a new mesonivirus, Yichang virus, in mosquitoes and its interference with representative flaviviruses. Guoguo Ye Yujuan Wang Xiaoyun Liu Qiannan Dong Quanxin Cai Zhiming Yuan Han Xia 2020-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008920 https://doaj.org/article/ec011a1da26b41ad80e9996410f0eb35 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008920 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008920 https://doaj.org/article/ec011a1da26b41ad80e9996410f0eb35 PLoS Neglected Tropical Diseases, Vol 14, Iss 11, p e0008920 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008920 2022-12-31T06:00:24Z Advances in technology have greatly stimulated the understanding of insect-specific viruses (ISVs). Unfortunately, most of these findings are based on sequencing technology, and laboratory data are scarce on the transmission dynamics of ISVs in nature and the potential effects of these viruses on arboviruses. Mesonivirus is a class of ISVs with a wide geographical distribution. Recently, our laboratory reported the isolation of a novel strain of mesonivirus, Yichang virus (YCV), from Culex mosquitoes, China. In this study, the experimental infection of YCV by the oral route for adult and larvae mosquitoes, and the vertical transmission has been conducted, which suggests that YCV could adopt a mixed-mode transmission. Controlled experiments showed that the infectivity of YCV depends on the mosquito species, virus dose, and infection route. The proliferation curve and tissue distribution of YCV in Cx. quinquefasciatus and Ae. albopictus showed that YCV is more susceptible to Ae. albopictus and is located in the midgut. Furthermore, we also assessed the interference of YCV with flaviviruses both in vitro and in vivo. YCV significantly inhibited the proliferation of DENV-2 and ZIKV, in cell culture, and reduced transmission rate of DENV-2 in Ae. albopictus. Our work provides insights into the transmission of ISVs in different mosquito species during ontogeny and their potential ability to interact with mosquito-borne viruses. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 11 e0008920 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Guoguo Ye Yujuan Wang Xiaoyun Liu Qiannan Dong Quanxin Cai Zhiming Yuan Han Xia Transmission competence of a new mesonivirus, Yichang virus, in mosquitoes and its interference with representative flaviviruses. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Advances in technology have greatly stimulated the understanding of insect-specific viruses (ISVs). Unfortunately, most of these findings are based on sequencing technology, and laboratory data are scarce on the transmission dynamics of ISVs in nature and the potential effects of these viruses on arboviruses. Mesonivirus is a class of ISVs with a wide geographical distribution. Recently, our laboratory reported the isolation of a novel strain of mesonivirus, Yichang virus (YCV), from Culex mosquitoes, China. In this study, the experimental infection of YCV by the oral route for adult and larvae mosquitoes, and the vertical transmission has been conducted, which suggests that YCV could adopt a mixed-mode transmission. Controlled experiments showed that the infectivity of YCV depends on the mosquito species, virus dose, and infection route. The proliferation curve and tissue distribution of YCV in Cx. quinquefasciatus and Ae. albopictus showed that YCV is more susceptible to Ae. albopictus and is located in the midgut. Furthermore, we also assessed the interference of YCV with flaviviruses both in vitro and in vivo. YCV significantly inhibited the proliferation of DENV-2 and ZIKV, in cell culture, and reduced transmission rate of DENV-2 in Ae. albopictus. Our work provides insights into the transmission of ISVs in different mosquito species during ontogeny and their potential ability to interact with mosquito-borne viruses. |
format |
Article in Journal/Newspaper |
author |
Guoguo Ye Yujuan Wang Xiaoyun Liu Qiannan Dong Quanxin Cai Zhiming Yuan Han Xia |
author_facet |
Guoguo Ye Yujuan Wang Xiaoyun Liu Qiannan Dong Quanxin Cai Zhiming Yuan Han Xia |
author_sort |
Guoguo Ye |
title |
Transmission competence of a new mesonivirus, Yichang virus, in mosquitoes and its interference with representative flaviviruses. |
title_short |
Transmission competence of a new mesonivirus, Yichang virus, in mosquitoes and its interference with representative flaviviruses. |
title_full |
Transmission competence of a new mesonivirus, Yichang virus, in mosquitoes and its interference with representative flaviviruses. |
title_fullStr |
Transmission competence of a new mesonivirus, Yichang virus, in mosquitoes and its interference with representative flaviviruses. |
title_full_unstemmed |
Transmission competence of a new mesonivirus, Yichang virus, in mosquitoes and its interference with representative flaviviruses. |
title_sort |
transmission competence of a new mesonivirus, yichang virus, in mosquitoes and its interference with representative flaviviruses. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008920 https://doaj.org/article/ec011a1da26b41ad80e9996410f0eb35 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 11, p e0008920 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008920 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008920 https://doaj.org/article/ec011a1da26b41ad80e9996410f0eb35 |
op_doi |
https://doi.org/10.1371/journal.pntd.0008920 |
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PLOS Neglected Tropical Diseases |
container_volume |
14 |
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11 |
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e0008920 |
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1766341167650177024 |