An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum

• Published in: Malaria Journal
• Main Authors: Hasenkamp Sandra, Wong Eleanor H, Horrocks Paul
• Format: Article in Journal/Newspaper
• Language: English
• Published: BMC 2012
• Subjects: Luciferase; Single-step lysis; Stage-specific; Promoter function; pcna; Arctic medicine. Tropical medicine; RC955-962; Infectious and parasitic diseases; RC109-216; Arctic
• Online Access: https://doi.org/10.1186/1475-2875-11-42; https://doaj.org/article/e85c8edb9609417da042f8ab351d6ebf

Abstract This report describes the optimization and evaluation of a simple single-step lysis protocol to measure luciferase bioluminescence from genetically modified Plasmodium falciparum . This protocol utilizes a modified commercial buffer to improve speed of assay and consistency in the bioluminescence signal measured by reducing the manipulation steps required to release the cytoplasmic fraction. The utility of this improved assay protocol is demonstrated in typical assays that explore absolute and temporal gene expression activity.