An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum
Abstract This report describes the optimization and evaluation of a simple single-step lysis protocol to measure luciferase bioluminescence from genetically modified Plasmodium falciparum . This protocol utilizes a modified commercial buffer to improve speed of assay and consistency in the biolumine...
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ftdoajarticles:oai:doaj.org/article:e85c8edb9609417da042f8ab351d6ebf 2023-05-15T14:58:59+02:00 An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum Hasenkamp Sandra Wong Eleanor H Horrocks Paul 2012-02-01T00:00:00Z https://doi.org/10.1186/1475-2875-11-42 https://doaj.org/article/e85c8edb9609417da042f8ab351d6ebf EN eng BMC http://www.malariajournal.com/content/11/1/42 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-42 1475-2875 https://doaj.org/article/e85c8edb9609417da042f8ab351d6ebf Malaria Journal, Vol 11, Iss 1, p 42 (2012) Luciferase Single-step lysis Stage-specific Promoter function pcna Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2012 ftdoajarticles https://doi.org/10.1186/1475-2875-11-42 2022-12-31T09:11:26Z Abstract This report describes the optimization and evaluation of a simple single-step lysis protocol to measure luciferase bioluminescence from genetically modified Plasmodium falciparum . This protocol utilizes a modified commercial buffer to improve speed of assay and consistency in the bioluminescence signal measured by reducing the manipulation steps required to release the cytoplasmic fraction. The utility of this improved assay protocol is demonstrated in typical assays that explore absolute and temporal gene expression activity. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 11 1 42 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Luciferase Single-step lysis Stage-specific Promoter function pcna Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Luciferase Single-step lysis Stage-specific Promoter function pcna Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Hasenkamp Sandra Wong Eleanor H Horrocks Paul An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum |
topic_facet |
Luciferase Single-step lysis Stage-specific Promoter function pcna Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract This report describes the optimization and evaluation of a simple single-step lysis protocol to measure luciferase bioluminescence from genetically modified Plasmodium falciparum . This protocol utilizes a modified commercial buffer to improve speed of assay and consistency in the bioluminescence signal measured by reducing the manipulation steps required to release the cytoplasmic fraction. The utility of this improved assay protocol is demonstrated in typical assays that explore absolute and temporal gene expression activity. |
format |
Article in Journal/Newspaper |
author |
Hasenkamp Sandra Wong Eleanor H Horrocks Paul |
author_facet |
Hasenkamp Sandra Wong Eleanor H Horrocks Paul |
author_sort |
Hasenkamp Sandra |
title |
An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum |
title_short |
An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum |
title_full |
An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum |
title_fullStr |
An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum |
title_full_unstemmed |
An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum |
title_sort |
improved single-step lysis protocol to measure luciferase bioluminescence in plasmodium falciparum |
publisher |
BMC |
publishDate |
2012 |
url |
https://doi.org/10.1186/1475-2875-11-42 https://doaj.org/article/e85c8edb9609417da042f8ab351d6ebf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 11, Iss 1, p 42 (2012) |
op_relation |
http://www.malariajournal.com/content/11/1/42 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-42 1475-2875 https://doaj.org/article/e85c8edb9609417da042f8ab351d6ebf |
op_doi |
https://doi.org/10.1186/1475-2875-11-42 |
container_title |
Malaria Journal |
container_volume |
11 |
container_issue |
1 |
container_start_page |
42 |
_version_ |
1766331097339133952 |