An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum

Abstract This report describes the optimization and evaluation of a simple single-step lysis protocol to measure luciferase bioluminescence from genetically modified Plasmodium falciparum . This protocol utilizes a modified commercial buffer to improve speed of assay and consistency in the biolumine...

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Bibliographic Details
Published in:Malaria Journal
Main Authors: Hasenkamp Sandra, Wong Eleanor H, Horrocks Paul
Format: Article in Journal/Newspaper
Language:English
Published: BMC 2012
Subjects:
Luciferase
Single-step lysis
Stage-specific
Promoter function
pcna
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Arctic
Online Access:https://doi.org/10.1186/1475-2875-11-42
https://doaj.org/article/e85c8edb9609417da042f8ab351d6ebf
Description
Summary:Abstract This report describes the optimization and evaluation of a simple single-step lysis protocol to measure luciferase bioluminescence from genetically modified Plasmodium falciparum . This protocol utilizes a modified commercial buffer to improve speed of assay and consistency in the bioluminescence signal measured by reducing the manipulation steps required to release the cytoplasmic fraction. The utility of this improved assay protocol is demonstrated in typical assays that explore absolute and temporal gene expression activity.

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