Three‐liquid‐phase salting‐out extraction of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)‐rich oils from Euphausia superba

• Published in: Engineering in Life Sciences
• Main Authors: Liaqat Zeb, Xin‐Nan Teng, Muhammad Shafiq, Shu‐Chang Wang, Zhi‐Long Xiu, Zhi‐Guo Su
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley-VCH 2021
• Subjects: Antarctic krill; docosahexaenoic acid; eicosapentaenoic acid; extraction efficiency; fluoride; three‐liquid‐phase salting‐out extraction system; Biotechnology; TP248.13-248.65; Antarctic; Antarc*; Antarctic Krill; Euphausia superba
• Online Access: https://doi.org/10.1002/elsc.202000098; https://doaj.org/article/e82068b0369443c9abdd0a73925e5fa9

Abstract The TLPSOES parameters were optimized by response surface methodology using Box–Behnken design, which were 16.5% w/w of ammonium citrate, 17.5% w/w of ethanol, and 46% w/w of n‐hexane at 70 min of stirring time. Under optimized conditions the extraction efficiency attained was 90.91 ± 0.97% of EPA, 90.02 ± 1.04% of DHA, and 91.85 ± 1.11% of KO in the top n‐hexane phase. The highest extraction efficiency of proteins and flavonoids, i.e. 88.34 ± 1.35% and 79.67 ± 1.13%, was recorded in the solid interface and ethanol phase, respectively. The KO extracted by TLPSOES system consisted of lowest fluoride level compared to the conventional method and whole wet krill biomass. The TLPSOES is a potential candidate for nutraceutical industry of KO extraction from wet krill biomass.