Isolation and Characterization of a Novel Sialoglycopeptide Promoting Osteogenesis from Gadus morhua Eggs
Gadus morhua eggs contain several nutrients, including polyunsaturated fatty acids, lecithin and glycoproteins. A novel sialoglycopeptide from the eggs of G. morhua ( Gm -SGPP) was extracted with 90% phenol and purified by Q Sepharose Fast Flow (QFF) ion exchange chromatography, followed by S-300 ge...
| Published in: | Molecules |
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| Main Authors: | , , , , , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
MDPI AG
2019
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| Subjects: | |
| Online Access: | https://doi.org/10.3390/molecules25010156 https://doaj.org/article/e79b89322640425586504e13907960cb |
| Summary: | Gadus morhua eggs contain several nutrients, including polyunsaturated fatty acids, lecithin and glycoproteins. A novel sialoglycopeptide from the eggs of G. morhua ( Gm -SGPP) was extracted with 90% phenol and purified by Q Sepharose Fast Flow (QFF) ion exchange chromatography, followed by S-300 gel filtration chromatography. Gm -SGPP contained 63.7% carbohydrate, 16.2% protein and 18.6% N-acetylneuraminic acid. High-performance size exclusion chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated that Gm -SGPP is a 7000-Da pure sialoglycopeptide. β-elimination reaction suggested that Gm -SGPP contained N-glycan units. Amino acid N-terminal sequence analysis indicated the presence of Ala-Ser-Asn-Gly-Thr-Gln-Ala-Pro amino acid sequence. Moreover, N-glycan was connected at the third Asn location of the peptide chain through GlcNAc. Gm -SGPP was composed of D-mannose, D-glucuronic acid and D-galactose. Fourier transform-infrared spectroscopy (FT-IR), 1 H-nuclear magnetic resonance spectroscopy ( 1 H-NMR) and methylation analysis were performed to reveal the structure profile of Gm -SGPP. In vitro results showed that the proliferation activity of MC3T3-E1 cells was significantly promoted by Gm -SGPP. In vivo data revealed that Gm -SGPP increased the calcium and phosphorus content of tibias and promoted longitudinal bone growth in adolescent rats. |
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