Deletion of Salmonella enterica serovar typhimurium sipC gene

Objective: To construct a novel plasmid as Salmonella enterica serovar typhimurium (S. typhimurium) sipC gene knockouts candidate. Methods: In this research, 5′ upstream and 3′ downstream regions of S. typhimurium sipC gene and kanamycin gene were PCR amplified. Each of these DNA fragment was cloned...

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Bibliographic Details
Published in:Asian Pacific Journal of Tropical Biomedicine
Main Authors: Maryam Safarpour Dehkordi, Abbas Doosti, Asghar Arshi
Format: Article in Journal/Newspaper
Language:English
Published: Wolters Kluwer Medknow Publications 2015
Subjects:
Salmonella enterica serovar typhimurium
sipC gene
TA cloning
Gene construct
pET-32 vector
Arctic medicine. Tropical medicine
RC955-962
Biology (General)
QH301-705.5
Arctic
Online Access:https://doi.org/10.1016/j.apjtb.2015.09.003
https://doaj.org/article/e54f31aad37645ddae310c0efeba7435
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Summary:Objective: To construct a novel plasmid as Salmonella enterica serovar typhimurium (S. typhimurium) sipC gene knockouts candidate. Methods: In this research, 5′ upstream and 3′ downstream regions of S. typhimurium sipC gene and kanamycin gene were PCR amplified. Each of these DNA fragment was cloned into pGEM T-easy vector. The construct was confirmed by PCR and restriction digest. Results: PCR amplified 320, 206 and 835 bp DNA fragments were subcloned into pET-32 vector resulting with a plasmid called pET-32-sipC up-kan- sip C down. Conclusions: The new plasmid (pET-32-sipC up-kan- sip C down) is useful for genetic engineering and for future manipulation of S. typhimurium sipC gene.

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