Deletion of Salmonella enterica serovar typhimurium sipC gene
Objective: To construct a novel plasmid as Salmonella enterica serovar typhimurium (S. typhimurium) sipC gene knockouts candidate. Methods: In this research, 5′ upstream and 3′ downstream regions of S. typhimurium sipC gene and kanamycin gene were PCR amplified. Each of these DNA fragment was cloned...
| Published in: | Asian Pacific Journal of Tropical Biomedicine |
|---|---|
| Main Authors: | , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2015
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| Subjects: | |
| Online Access: | https://doi.org/10.1016/j.apjtb.2015.09.003 https://doaj.org/article/e54f31aad37645ddae310c0efeba7435 |
| _version_ | 1821829355759730688 |
|---|---|
| author | Maryam Safarpour Dehkordi Abbas Doosti Asghar Arshi |
| author_facet | Maryam Safarpour Dehkordi Abbas Doosti Asghar Arshi |
| author_sort | Maryam Safarpour Dehkordi |
| collection | Directory of Open Access Journals: DOAJ Articles |
| container_issue | 12 |
| container_start_page | 987 |
| container_title | Asian Pacific Journal of Tropical Biomedicine |
| container_volume | 5 |
| description | Objective: To construct a novel plasmid as Salmonella enterica serovar typhimurium (S. typhimurium) sipC gene knockouts candidate. Methods: In this research, 5′ upstream and 3′ downstream regions of S. typhimurium sipC gene and kanamycin gene were PCR amplified. Each of these DNA fragment was cloned into pGEM T-easy vector. The construct was confirmed by PCR and restriction digest. Results: PCR amplified 320, 206 and 835 bp DNA fragments were subcloned into pET-32 vector resulting with a plasmid called pET-32-sipC up-kan- sip C down. Conclusions: The new plasmid (pET-32-sipC up-kan- sip C down) is useful for genetic engineering and for future manipulation of S. typhimurium sipC gene. |
| format | Article in Journal/Newspaper |
| genre | Arctic |
| genre_facet | Arctic |
| geographic | Arctic |
| geographic_facet | Arctic |
| id | ftdoajarticles:oai:doaj.org/article:e54f31aad37645ddae310c0efeba7435 |
| institution | Open Polar |
| language | English |
| op_collection_id | ftdoajarticles |
| op_container_end_page | 991 |
| op_doi | https://doi.org/10.1016/j.apjtb.2015.09.003 |
| op_relation | http://www.sciencedirect.com/science/article/pii/S2221169115002154 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2015.09.003 https://doaj.org/article/e54f31aad37645ddae310c0efeba7435 |
| op_source | Asian Pacific Journal of Tropical Biomedicine, Vol 5, Iss 12, Pp 987-991 (2015) |
| publishDate | 2015 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | openpolar |
| spelling | ftdoajarticles:oai:doaj.org/article:e54f31aad37645ddae310c0efeba7435 2025-01-16T20:34:19+00:00 Deletion of Salmonella enterica serovar typhimurium sipC gene Maryam Safarpour Dehkordi Abbas Doosti Asghar Arshi 2015-12-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2015.09.003 https://doaj.org/article/e54f31aad37645ddae310c0efeba7435 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115002154 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2015.09.003 https://doaj.org/article/e54f31aad37645ddae310c0efeba7435 Asian Pacific Journal of Tropical Biomedicine, Vol 5, Iss 12, Pp 987-991 (2015) Salmonella enterica serovar typhimurium sipC gene TA cloning Gene construct pET-32 vector Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2015 ftdoajarticles https://doi.org/10.1016/j.apjtb.2015.09.003 2022-12-31T14:05:37Z Objective: To construct a novel plasmid as Salmonella enterica serovar typhimurium (S. typhimurium) sipC gene knockouts candidate. Methods: In this research, 5′ upstream and 3′ downstream regions of S. typhimurium sipC gene and kanamycin gene were PCR amplified. Each of these DNA fragment was cloned into pGEM T-easy vector. The construct was confirmed by PCR and restriction digest. Results: PCR amplified 320, 206 and 835 bp DNA fragments were subcloned into pET-32 vector resulting with a plasmid called pET-32-sipC up-kan- sip C down. Conclusions: The new plasmid (pET-32-sipC up-kan- sip C down) is useful for genetic engineering and for future manipulation of S. typhimurium sipC gene. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 5 12 987 991 |
| spellingShingle | Salmonella enterica serovar typhimurium sipC gene TA cloning Gene construct pET-32 vector Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Maryam Safarpour Dehkordi Abbas Doosti Asghar Arshi Deletion of Salmonella enterica serovar typhimurium sipC gene |
| title | Deletion of Salmonella enterica serovar typhimurium sipC gene |
| title_full | Deletion of Salmonella enterica serovar typhimurium sipC gene |
| title_fullStr | Deletion of Salmonella enterica serovar typhimurium sipC gene |
| title_full_unstemmed | Deletion of Salmonella enterica serovar typhimurium sipC gene |
| title_short | Deletion of Salmonella enterica serovar typhimurium sipC gene |
| title_sort | deletion of salmonella enterica serovar typhimurium sipc gene |
| topic | Salmonella enterica serovar typhimurium sipC gene TA cloning Gene construct pET-32 vector Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
| topic_facet | Salmonella enterica serovar typhimurium sipC gene TA cloning Gene construct pET-32 vector Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
| url | https://doi.org/10.1016/j.apjtb.2015.09.003 https://doaj.org/article/e54f31aad37645ddae310c0efeba7435 |