Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov.
A group of seven bacterial strains producing blue-purple pigmented colonies on R2A agar was isolated from freshwater samples collected in a deglaciated part of James Ross Island and Eagle Island, Antarctica, from 2017–2019. The isolates were psychrophilic, oligotrophic, resistant to chloramphenicol,...
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ftdoajarticles:oai:doaj.org/article:e37ea82c2fce4efb8464e420e13529b1 2024-01-07T09:39:51+01:00 Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov. Ivo Sedláček Pavla Holochová Hans-Jürgen Busse Vendula Koublová Stanislava Králová Pavel Švec Roman Sobotka Eva Staňková Jan Pilný Ondrej Šedo Jana Smolíková Karel Sedlář 2022-03-01T00:00:00Z https://doi.org/10.3390/microorganisms10040704 https://doaj.org/article/e37ea82c2fce4efb8464e420e13529b1 EN eng MDPI AG https://www.mdpi.com/2076-2607/10/4/704 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms10040704 2076-2607 https://doaj.org/article/e37ea82c2fce4efb8464e420e13529b1 Microorganisms, Vol 10, Iss 4, p 704 (2022) Massilia violacein psychrophilic description whole-genome sequencing Antarctica Biology (General) QH301-705.5 article 2022 ftdoajarticles https://doi.org/10.3390/microorganisms10040704 2023-12-10T01:44:50Z A group of seven bacterial strains producing blue-purple pigmented colonies on R2A agar was isolated from freshwater samples collected in a deglaciated part of James Ross Island and Eagle Island, Antarctica, from 2017–2019. The isolates were psychrophilic, oligotrophic, resistant to chloramphenicol, and exhibited strong hydrolytic activities. To clarify the taxonomic position of these isolates, a polyphasic taxonomic approach was applied based on sequencing of the 16S rRNA, gyr B and lep A genes, whole-genome sequencing, rep-PCR, MALDI-TOF MS, chemotaxonomy analyses and biotyping. Phylogenetic analysis of the 16S rRNA gene sequences revealed that the entire group are representatives of the genus Massilia . The closest relatives of the reference strain P8398 T were Massilia atriviolacea , Massilia violaceinigra , Massilia rubra , Massilia mucilaginosa , Massilia aquatica , Massilia frigida , Massilia glaciei and Massilia eurypsychrophila with a pairwise similarity of 98.6–100% in the 16S rRNA. The subsequent gyr B and lep A sequencing results showed the novelty of the analysed group, and the average nucleotide identity and digital DNA–DNA hybridisation values clearly proved that P8398 T represents a distinct Massilia species. After all these results, we nominate a new species with the proposed name Massilia antarctica sp. nov. The type strain is P8398 T (= CCM 8941 T = LMG 32108 T ). Article in Journal/Newspaper Antarc* Antarctica Eagle Island James Ross Island Ross Island Directory of Open Access Journals: DOAJ Articles Eagle Island ENVELOPE(-57.489,-57.489,-63.660,-63.660) Ross Island Microorganisms 10 4 704 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Massilia violacein psychrophilic description whole-genome sequencing Antarctica Biology (General) QH301-705.5 |
spellingShingle |
Massilia violacein psychrophilic description whole-genome sequencing Antarctica Biology (General) QH301-705.5 Ivo Sedláček Pavla Holochová Hans-Jürgen Busse Vendula Koublová Stanislava Králová Pavel Švec Roman Sobotka Eva Staňková Jan Pilný Ondrej Šedo Jana Smolíková Karel Sedlář Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov. |
topic_facet |
Massilia violacein psychrophilic description whole-genome sequencing Antarctica Biology (General) QH301-705.5 |
description |
A group of seven bacterial strains producing blue-purple pigmented colonies on R2A agar was isolated from freshwater samples collected in a deglaciated part of James Ross Island and Eagle Island, Antarctica, from 2017–2019. The isolates were psychrophilic, oligotrophic, resistant to chloramphenicol, and exhibited strong hydrolytic activities. To clarify the taxonomic position of these isolates, a polyphasic taxonomic approach was applied based on sequencing of the 16S rRNA, gyr B and lep A genes, whole-genome sequencing, rep-PCR, MALDI-TOF MS, chemotaxonomy analyses and biotyping. Phylogenetic analysis of the 16S rRNA gene sequences revealed that the entire group are representatives of the genus Massilia . The closest relatives of the reference strain P8398 T were Massilia atriviolacea , Massilia violaceinigra , Massilia rubra , Massilia mucilaginosa , Massilia aquatica , Massilia frigida , Massilia glaciei and Massilia eurypsychrophila with a pairwise similarity of 98.6–100% in the 16S rRNA. The subsequent gyr B and lep A sequencing results showed the novelty of the analysed group, and the average nucleotide identity and digital DNA–DNA hybridisation values clearly proved that P8398 T represents a distinct Massilia species. After all these results, we nominate a new species with the proposed name Massilia antarctica sp. nov. The type strain is P8398 T (= CCM 8941 T = LMG 32108 T ). |
format |
Article in Journal/Newspaper |
author |
Ivo Sedláček Pavla Holochová Hans-Jürgen Busse Vendula Koublová Stanislava Králová Pavel Švec Roman Sobotka Eva Staňková Jan Pilný Ondrej Šedo Jana Smolíková Karel Sedlář |
author_facet |
Ivo Sedláček Pavla Holochová Hans-Jürgen Busse Vendula Koublová Stanislava Králová Pavel Švec Roman Sobotka Eva Staňková Jan Pilný Ondrej Šedo Jana Smolíková Karel Sedlář |
author_sort |
Ivo Sedláček |
title |
Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov. |
title_short |
Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov. |
title_full |
Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov. |
title_fullStr |
Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov. |
title_full_unstemmed |
Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov. |
title_sort |
characterisation of waterborne psychrophilic massilia isolates with violacein production and description of massilia antarctica sp. nov. |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/microorganisms10040704 https://doaj.org/article/e37ea82c2fce4efb8464e420e13529b1 |
long_lat |
ENVELOPE(-57.489,-57.489,-63.660,-63.660) |
geographic |
Eagle Island Ross Island |
geographic_facet |
Eagle Island Ross Island |
genre |
Antarc* Antarctica Eagle Island James Ross Island Ross Island |
genre_facet |
Antarc* Antarctica Eagle Island James Ross Island Ross Island |
op_source |
Microorganisms, Vol 10, Iss 4, p 704 (2022) |
op_relation |
https://www.mdpi.com/2076-2607/10/4/704 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms10040704 2076-2607 https://doaj.org/article/e37ea82c2fce4efb8464e420e13529b1 |
op_doi |
https://doi.org/10.3390/microorganisms10040704 |
container_title |
Microorganisms |
container_volume |
10 |
container_issue |
4 |
container_start_page |
704 |
_version_ |
1787430123255889920 |