Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov.

A group of seven bacterial strains producing blue-purple pigmented colonies on R2A agar was isolated from freshwater samples collected in a deglaciated part of James Ross Island and Eagle Island, Antarctica, from 2017–2019. The isolates were psychrophilic, oligotrophic, resistant to chloramphenicol,...

Full description

Bibliographic Details
Published in:Microorganisms
Main Authors: Ivo Sedláček, Pavla Holochová, Hans-Jürgen Busse, Vendula Koublová, Stanislava Králová, Pavel Švec, Roman Sobotka, Eva Staňková, Jan Pilný, Ondrej Šedo, Jana Smolíková, Karel Sedlář
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2022
Subjects:
Massilia
violacein
psychrophilic
description
whole-genome sequencing
Antarctica
Biology (General)
QH301-705.5
Eagle Island
Ross Island
Antarc*
James Ross Island
Online Access:https://doi.org/10.3390/microorganisms10040704
https://doaj.org/article/e37ea82c2fce4efb8464e420e13529b1
Description
Summary:A group of seven bacterial strains producing blue-purple pigmented colonies on R2A agar was isolated from freshwater samples collected in a deglaciated part of James Ross Island and Eagle Island, Antarctica, from 2017–2019. The isolates were psychrophilic, oligotrophic, resistant to chloramphenicol, and exhibited strong hydrolytic activities. To clarify the taxonomic position of these isolates, a polyphasic taxonomic approach was applied based on sequencing of the 16S rRNA, gyr B and lep A genes, whole-genome sequencing, rep-PCR, MALDI-TOF MS, chemotaxonomy analyses and biotyping. Phylogenetic analysis of the 16S rRNA gene sequences revealed that the entire group are representatives of the genus Massilia . The closest relatives of the reference strain P8398 T were Massilia atriviolacea , Massilia violaceinigra , Massilia rubra , Massilia mucilaginosa , Massilia aquatica , Massilia frigida , Massilia glaciei and Massilia eurypsychrophila with a pairwise similarity of 98.6–100% in the 16S rRNA. The subsequent gyr B and lep A sequencing results showed the novelty of the analysed group, and the average nucleotide identity and digital DNA–DNA hybridisation values clearly proved that P8398 T represents a distinct Massilia species. After all these results, we nominate a new species with the proposed name Massilia antarctica sp. nov. The type strain is P8398 T (= CCM 8941 T = LMG 32108 T ).

Similar Items