Investigation of Enteric Viruses in the Feces of Neotropical Migratory Birds Captured on the Coast of the State of Pará, Brazil
ABSTRACT Migratory birds can become long-distance vectors for a wide range of microorganisms and can cause human disease, being the Brazilian coast a gateway for northern migratory birds. These animals are considered natural reservoirs of different viruses that cause important diseases, being releva...
Published in: | Revista Brasileira de Ciência Avícola |
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Online Access: | https://doi.org/10.1590/1806-9061-2017-0589 https://doaj.org/article/e2eb6026ca9c4f4b95ddebe6a1aceb8c |
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ftdoajarticles:oai:doaj.org/article:e2eb6026ca9c4f4b95ddebe6a1aceb8c 2023-05-15T17:47:21+02:00 Investigation of Enteric Viruses in the Feces of Neotropical Migratory Birds Captured on the Coast of the State of Pará, Brazil AN Guerreiro CCG Moraes ANR Marinho BCV Barros DAM Bezerra RS Bandeira RR Silva DCC Rocha AMC Meneses MA Luz GS Paz JDP Mascarenhas https://doi.org/10.1590/1806-9061-2017-0589 https://doaj.org/article/e2eb6026ca9c4f4b95ddebe6a1aceb8c EN eng Fundação APINCO de Ciência e Tecnologia Avícolas http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000100161&lng=en&tlng=en https://doaj.org/toc/1806-9061 1806-9061 doi:10.1590/1806-9061-2017-0589 https://doaj.org/article/e2eb6026ca9c4f4b95ddebe6a1aceb8c Brazilian Journal of Poultry Science, Vol 20, Iss 1, Pp 161-168 Animals Amazônia picobirnavirus reovirus rotavirus RT-PCR Animal culture SF1-1100 Veterinary medicine SF600-1100 Zoology QL1-991 article ftdoajarticles https://doi.org/10.1590/1806-9061-2017-0589 2022-12-31T01:05:42Z ABSTRACT Migratory birds can become long-distance vectors for a wide range of microorganisms and can cause human disease, being the Brazilian coast a gateway for northern migratory birds. These animals are considered natural reservoirs of different viruses that cause important diseases, being relevant research of viral pathogens in migratory birds to epidemiology surveillance. The objective of the study was to investigate the presence of avian rotavirus (AvRV), avian reovirus (ARV) and picobirnavirus (PBV) in Neotropical migratory birds captured on the coast of Brazil. A total of 23 individual fecal samples of the migratory birds species Calidris pusilla (20 birds), Numenius phaeopus (1 bird) and Charadrius semipalmatus (2 birds) were collected. Fecal suspensions were prepared from the collected samples for subsequent extraction of double-stranded RNA (dsRNA), which was subjected to polyacrylamide gel electrophoresis (PAGE) and reverse transcription polymerase chain reaction (RT-PCR). The electrophoretic profiles were not detected by PAGE, and the amplification for the studied viruses PBV, ARV and AvRV (specie D, gene VP6 and NSP4) were negative. Positivity for AvRVD, VP7 gene was of 4.35% (1/23) for the migratory bird Calidris pusilla. After sequencing and building the tree of phylogenetic relationships avian Rotavirus Group D identified in this study was phylogenetically related and grouped into one branch, together to previously reported AvRVD from Brazil in chicken flocks with 99.8% nucleotide and 100% amino acid similarities. Article in Journal/Newspaper Numenius phaeopus Directory of Open Access Journals: DOAJ Articles Revista Brasileira de Ciência Avícola 20 1 161 168 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Animals Amazônia picobirnavirus reovirus rotavirus RT-PCR Animal culture SF1-1100 Veterinary medicine SF600-1100 Zoology QL1-991 |
spellingShingle |
Animals Amazônia picobirnavirus reovirus rotavirus RT-PCR Animal culture SF1-1100 Veterinary medicine SF600-1100 Zoology QL1-991 AN Guerreiro CCG Moraes ANR Marinho BCV Barros DAM Bezerra RS Bandeira RR Silva DCC Rocha AMC Meneses MA Luz GS Paz JDP Mascarenhas Investigation of Enteric Viruses in the Feces of Neotropical Migratory Birds Captured on the Coast of the State of Pará, Brazil |
topic_facet |
Animals Amazônia picobirnavirus reovirus rotavirus RT-PCR Animal culture SF1-1100 Veterinary medicine SF600-1100 Zoology QL1-991 |
description |
ABSTRACT Migratory birds can become long-distance vectors for a wide range of microorganisms and can cause human disease, being the Brazilian coast a gateway for northern migratory birds. These animals are considered natural reservoirs of different viruses that cause important diseases, being relevant research of viral pathogens in migratory birds to epidemiology surveillance. The objective of the study was to investigate the presence of avian rotavirus (AvRV), avian reovirus (ARV) and picobirnavirus (PBV) in Neotropical migratory birds captured on the coast of Brazil. A total of 23 individual fecal samples of the migratory birds species Calidris pusilla (20 birds), Numenius phaeopus (1 bird) and Charadrius semipalmatus (2 birds) were collected. Fecal suspensions were prepared from the collected samples for subsequent extraction of double-stranded RNA (dsRNA), which was subjected to polyacrylamide gel electrophoresis (PAGE) and reverse transcription polymerase chain reaction (RT-PCR). The electrophoretic profiles were not detected by PAGE, and the amplification for the studied viruses PBV, ARV and AvRV (specie D, gene VP6 and NSP4) were negative. Positivity for AvRVD, VP7 gene was of 4.35% (1/23) for the migratory bird Calidris pusilla. After sequencing and building the tree of phylogenetic relationships avian Rotavirus Group D identified in this study was phylogenetically related and grouped into one branch, together to previously reported AvRVD from Brazil in chicken flocks with 99.8% nucleotide and 100% amino acid similarities. |
format |
Article in Journal/Newspaper |
author |
AN Guerreiro CCG Moraes ANR Marinho BCV Barros DAM Bezerra RS Bandeira RR Silva DCC Rocha AMC Meneses MA Luz GS Paz JDP Mascarenhas |
author_facet |
AN Guerreiro CCG Moraes ANR Marinho BCV Barros DAM Bezerra RS Bandeira RR Silva DCC Rocha AMC Meneses MA Luz GS Paz JDP Mascarenhas |
author_sort |
AN Guerreiro |
title |
Investigation of Enteric Viruses in the Feces of Neotropical Migratory Birds Captured on the Coast of the State of Pará, Brazil |
title_short |
Investigation of Enteric Viruses in the Feces of Neotropical Migratory Birds Captured on the Coast of the State of Pará, Brazil |
title_full |
Investigation of Enteric Viruses in the Feces of Neotropical Migratory Birds Captured on the Coast of the State of Pará, Brazil |
title_fullStr |
Investigation of Enteric Viruses in the Feces of Neotropical Migratory Birds Captured on the Coast of the State of Pará, Brazil |
title_full_unstemmed |
Investigation of Enteric Viruses in the Feces of Neotropical Migratory Birds Captured on the Coast of the State of Pará, Brazil |
title_sort |
investigation of enteric viruses in the feces of neotropical migratory birds captured on the coast of the state of pará, brazil |
publisher |
Fundação APINCO de Ciência e Tecnologia Avícolas |
url |
https://doi.org/10.1590/1806-9061-2017-0589 https://doaj.org/article/e2eb6026ca9c4f4b95ddebe6a1aceb8c |
genre |
Numenius phaeopus |
genre_facet |
Numenius phaeopus |
op_source |
Brazilian Journal of Poultry Science, Vol 20, Iss 1, Pp 161-168 |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000100161&lng=en&tlng=en https://doaj.org/toc/1806-9061 1806-9061 doi:10.1590/1806-9061-2017-0589 https://doaj.org/article/e2eb6026ca9c4f4b95ddebe6a1aceb8c |
op_doi |
https://doi.org/10.1590/1806-9061-2017-0589 |
container_title |
Revista Brasileira de Ciência Avícola |
container_volume |
20 |
container_issue |
1 |
container_start_page |
161 |
op_container_end_page |
168 |
_version_ |
1766151738474102784 |