Investigation of Enteric Viruses in the Feces of Neotropical Migratory Birds Captured on the Coast of the State of Pará, Brazil

ABSTRACT Migratory birds can become long-distance vectors for a wide range of microorganisms and can cause human disease, being the Brazilian coast a gateway for northern migratory birds. These animals are considered natural reservoirs of different viruses that cause important diseases, being releva...

Full description

Bibliographic Details
Published in:Revista Brasileira de Ciência Avícola
Main Authors: AN Guerreiro, CCG Moraes, ANR Marinho, BCV Barros, DAM Bezerra, RS Bandeira, RR Silva, DCC Rocha, AMC Meneses, MA Luz, GS Paz, JDP Mascarenhas
Format: Article in Journal/Newspaper
Language:English
Published: Fundação APINCO de Ciência e Tecnologia Avícolas
Subjects:
Animals
Amazônia
picobirnavirus
reovirus
rotavirus
RT-PCR
Animal culture
SF1-1100
Veterinary medicine
SF600-1100
Zoology
QL1-991
Numenius phaeopus
Online Access:https://doi.org/10.1590/1806-9061-2017-0589
https://doaj.org/article/e2eb6026ca9c4f4b95ddebe6a1aceb8c
Description
Summary:ABSTRACT Migratory birds can become long-distance vectors for a wide range of microorganisms and can cause human disease, being the Brazilian coast a gateway for northern migratory birds. These animals are considered natural reservoirs of different viruses that cause important diseases, being relevant research of viral pathogens in migratory birds to epidemiology surveillance. The objective of the study was to investigate the presence of avian rotavirus (AvRV), avian reovirus (ARV) and picobirnavirus (PBV) in Neotropical migratory birds captured on the coast of Brazil. A total of 23 individual fecal samples of the migratory birds species Calidris pusilla (20 birds), Numenius phaeopus (1 bird) and Charadrius semipalmatus (2 birds) were collected. Fecal suspensions were prepared from the collected samples for subsequent extraction of double-stranded RNA (dsRNA), which was subjected to polyacrylamide gel electrophoresis (PAGE) and reverse transcription polymerase chain reaction (RT-PCR). The electrophoretic profiles were not detected by PAGE, and the amplification for the studied viruses PBV, ARV and AvRV (specie D, gene VP6 and NSP4) were negative. Positivity for AvRVD, VP7 gene was of 4.35% (1/23) for the migratory bird Calidris pusilla. After sequencing and building the tree of phylogenetic relationships avian Rotavirus Group D identified in this study was phylogenetically related and grouped into one branch, together to previously reported AvRVD from Brazil in chicken flocks with 99.8% nucleotide and 100% amino acid similarities.

Similar Items