Point-of-care laboratory of pathogen diagnosis in rural Senegal.
BACKGROUND: In tropical Africa, where the spectrum of the bacterial pathogens that cause fevers is poorly understood and molecular-based diagnostic laboratories are rare, the time lag between test results and patient care is a critical point for treatment of disease. METHODOLOGY/PRINCIPAL FINDINGS:...
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ftdoajarticles:oai:doaj.org/article:e0a859733ff84b5c88284520cd2a4a3b 2023-05-15T15:12:58+02:00 Point-of-care laboratory of pathogen diagnosis in rural Senegal. Cheikh Sokhna Oleg Mediannikov Florence Fenollar Hubert Bassene Georges Diatta Adama Tall Jean-François Trape Michel Drancourt Didier Raoult 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001999 https://doaj.org/article/e0a859733ff84b5c88284520cd2a4a3b EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3547848?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001999 https://doaj.org/article/e0a859733ff84b5c88284520cd2a4a3b PLoS Neglected Tropical Diseases, Vol 7, Iss 1, p e1999 (2013) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001999 2022-12-31T00:08:49Z BACKGROUND: In tropical Africa, where the spectrum of the bacterial pathogens that cause fevers is poorly understood and molecular-based diagnostic laboratories are rare, the time lag between test results and patient care is a critical point for treatment of disease. METHODOLOGY/PRINCIPAL FINDINGS: We implemented POC laboratory in rural Senegal to resolve the time lag between test results and patient care. During the first year of the study (February 2011 to January 2012), 440 blood specimens from febrile patients were collected in Dielmo and Ndiop villages. All samples were screened for malaria, dengue fever, Borrelia spp., Coxiella burnetii, Tropheryma whipplei, Rickettsia conorii, R. africae, R. felis, and Bartonella spp. CONCLUSIONS/SIGNIFICANCE: We identified DNA from at least one pathogenic bacterium in 80/440 (18.2%) of the samples from febrile patients. B. crocidurae was identified in 35 cases (9.5%), and R. felis DNA was found in 30 cases (6.8%). The DNA of Bartonella spp. was identified in 23/440 cases (4.3%), and DNA of C. burnetii was identified in 2 cases (0.5%). T. whipplei (0.2%) was diagnosed in one patient. No DNA of R. africae or R. conorii was identified. Among the 7 patients co-infected by two different bacteria, we found R. felis and B. crocidurae in 4 cases, B. crocidurae and Bartonella spp. in 2 cases, and B. crocidurae and C. burnetii in 1 case. Malaria was diagnosed in 54 cases. In total, at least one pathogen (bacterium or protozoa) was identified in 127/440 (28.9%) of studied samples. Here, the authors report the proof of concept of POC in rural tropical Africa. Discovering that 18.2% of acute infections can be successfully treated with doxycycline should change the treatment strategy for acute fevers in West Africa. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 7 1 e1999 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Cheikh Sokhna Oleg Mediannikov Florence Fenollar Hubert Bassene Georges Diatta Adama Tall Jean-François Trape Michel Drancourt Didier Raoult Point-of-care laboratory of pathogen diagnosis in rural Senegal. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: In tropical Africa, where the spectrum of the bacterial pathogens that cause fevers is poorly understood and molecular-based diagnostic laboratories are rare, the time lag between test results and patient care is a critical point for treatment of disease. METHODOLOGY/PRINCIPAL FINDINGS: We implemented POC laboratory in rural Senegal to resolve the time lag between test results and patient care. During the first year of the study (February 2011 to January 2012), 440 blood specimens from febrile patients were collected in Dielmo and Ndiop villages. All samples were screened for malaria, dengue fever, Borrelia spp., Coxiella burnetii, Tropheryma whipplei, Rickettsia conorii, R. africae, R. felis, and Bartonella spp. CONCLUSIONS/SIGNIFICANCE: We identified DNA from at least one pathogenic bacterium in 80/440 (18.2%) of the samples from febrile patients. B. crocidurae was identified in 35 cases (9.5%), and R. felis DNA was found in 30 cases (6.8%). The DNA of Bartonella spp. was identified in 23/440 cases (4.3%), and DNA of C. burnetii was identified in 2 cases (0.5%). T. whipplei (0.2%) was diagnosed in one patient. No DNA of R. africae or R. conorii was identified. Among the 7 patients co-infected by two different bacteria, we found R. felis and B. crocidurae in 4 cases, B. crocidurae and Bartonella spp. in 2 cases, and B. crocidurae and C. burnetii in 1 case. Malaria was diagnosed in 54 cases. In total, at least one pathogen (bacterium or protozoa) was identified in 127/440 (28.9%) of studied samples. Here, the authors report the proof of concept of POC in rural tropical Africa. Discovering that 18.2% of acute infections can be successfully treated with doxycycline should change the treatment strategy for acute fevers in West Africa. |
format |
Article in Journal/Newspaper |
author |
Cheikh Sokhna Oleg Mediannikov Florence Fenollar Hubert Bassene Georges Diatta Adama Tall Jean-François Trape Michel Drancourt Didier Raoult |
author_facet |
Cheikh Sokhna Oleg Mediannikov Florence Fenollar Hubert Bassene Georges Diatta Adama Tall Jean-François Trape Michel Drancourt Didier Raoult |
author_sort |
Cheikh Sokhna |
title |
Point-of-care laboratory of pathogen diagnosis in rural Senegal. |
title_short |
Point-of-care laboratory of pathogen diagnosis in rural Senegal. |
title_full |
Point-of-care laboratory of pathogen diagnosis in rural Senegal. |
title_fullStr |
Point-of-care laboratory of pathogen diagnosis in rural Senegal. |
title_full_unstemmed |
Point-of-care laboratory of pathogen diagnosis in rural Senegal. |
title_sort |
point-of-care laboratory of pathogen diagnosis in rural senegal. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pntd.0001999 https://doaj.org/article/e0a859733ff84b5c88284520cd2a4a3b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 7, Iss 1, p e1999 (2013) |
op_relation |
http://europepmc.org/articles/PMC3547848?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001999 https://doaj.org/article/e0a859733ff84b5c88284520cd2a4a3b |
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https://doi.org/10.1371/journal.pntd.0001999 |
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PLoS Neglected Tropical Diseases |
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7 |
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1 |
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e1999 |
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