Purification and parcial characterization of a hemagglutinating factor (HAF): a possible adhesive factor of the diffuse adherent of Escherichia coli (DAEC) Purificação e caracterização parcial de um fator hemaglutinante (HAF): uma possível adesina de Escherichia coli do tipo aderência difusa (DAEC)

The mannose-resistant hemagglutinating factor (HAF) was extracted and purified from a diffuse adherent Escherichia coli (DAEC) strain belonging to the classic enteropathogenic E. coli (EPEC) serotype (0128). The molecular weight of HAF was estimated to be 18 KDa by SDS-PAGE and 66 KDa by Sephadex G1...

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Bibliographic Details
Published in:Revista do Instituto de Medicina Tropical de São Paulo
Main Authors: Yano Tomomasa, Cleide Ferreira Catani, Michiko Arita, Takeshi Honda, Toshio Miwatani
Format: Article in Journal/Newspaper
Language:English
Published: Universidade de São Paulo (USP) 1996
Subjects:
Escherichia coli
Diffuse adherence
Hemagglutinating factor (HAF)
adhesive factor
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Arctic
Haf
Online Access:https://doi.org/10.1590/S0036-46651996000600003
https://doaj.org/article/e034e440c1fe42358e9b9c1dc1fb7755
Description
Summary:The mannose-resistant hemagglutinating factor (HAF) was extracted and purified from a diffuse adherent Escherichia coli (DAEC) strain belonging to the classic enteropathogenic E. coli (EPEC) serotype (0128). The molecular weight of HAF was estimated to be 18 KDa by SDS-PAGE and 66 KDa by Sephadex G100, suggesting that the native form of HAF consists of 3-4 monomeric HAF. Gold immunolabeling with specific HAF antiserum revealed that the HAF is not a rigid structure like fimbriae on the bacterial surface. The immunofluorescence test using purified HAF on HeLa cells, in addition to the fact that the HAF is distributed among serotypes of EPEC, suggests that HAF is a possible adhesive factor of DAEC strains O fator hemaglutinante (HAF) foi extraído e purificado a partir de amostra de Escherichia coli de aderência difusa (DAEC), pertencente ao sorogrupo 0128 de E. coli enteropatogênica clássica (EPEC). O peso molecular do HAF foi estimado cm 18.000 Da usando SDS-PAGE e 66.000 Da empregando Sephadex G 100, o que sugere a estrutura do HAF consistir de 3 a 4 subunidades. O emprego da técnica "gold immunolabeling" com antissoro específico anti-HAF revelou que este fator não é uma estrutura do tipo fímbria na superfície da bactéria. O resultado do teste de imunofluorescência usando HAF purificado em células HeLa, além do fato deste fator estar presente entre os sorotipos de EPEC, sugerem que o HAF seja um possível fator de aderência das amostras de DAEC.

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