Nanopore Sequencing and De Novo Assembly of a Black-Shelled Pacific Oyster (Crassostrea gigas) Genome

The Pacific oyster, Crassostrea gigas, belongs to one of the most species-rich phyla and provides important ecological and economical services. Here we present a genome assembly for a variety of this species, black-shelled Pacific oyster, using a combination of 61.8 Gb Nanopore long reads and 105.6...

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Bibliographic Details
Published in:Frontiers in Genetics
Main Authors: Xiaotong Wang, Wenjie Xu, Lei Wei, Chenglong Zhu, Cheng He, Hongce Song, Zhongqiang Cai, Wenchao Yu, Qiuyun Jiang, Lingling Li, Kun Wang, Chenguang Feng
Format: Article in Journal/Newspaper
Language:English
Published: Frontiers Media S.A. 2019
Subjects:
Crassostrea gigas
black-shelled pacific oyster
evolution
genome assembly
nanopore sequencing
Genetics
QH426-470
Pacific
Pacific oyster
Online Access:https://doi.org/10.3389/fgene.2019.01211
https://doaj.org/article/deb06d266a65437fa9a2b9950c0df702
Description
Summary:The Pacific oyster, Crassostrea gigas, belongs to one of the most species-rich phyla and provides important ecological and economical services. Here we present a genome assembly for a variety of this species, black-shelled Pacific oyster, using a combination of 61.8 Gb Nanopore long reads and 105.6 Gb raw BGI-seq short reads. The genome assembly comprised 3,676 contigs, with a total length of 587 Mb and a contig N50 of 581 kb. Annotation of the genome assembly identified 283 Mb (48.32%) of repetitive sequences and a total of 26,811 protein-coding genes. A long-term transposable element active, accompanied by recent expansion (1 million years ago), was detected in this genome. The divergence between black-shelled and the previous published Pacific oysters was estimated at about 2.2 million years ago, which implies that species C. gigas had great intraspecific genetic variations. Moreover, we identified 148/188 specifically expanded/contracted gene families in this genome. We believe this genome assembly will be a valuable resource for understanding the genetic breeding, conservation, and evolution of oysters and bivalves.

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