The Comparison of PCR Kits for the Detection of Erythrocytic Parasites on Filter Paper

• Published in: Journal of Tropical Medicine
• Main Authors: Zhi-yong Tao, Pei-yi Zhang, Lu Zhang, Chun-cao Li, Rui Hu, Han-wu Zhu, Bei Zhou, Kai Wu, Ling-xu Li, Da-wei Yao, Yu-jie Cao, Dao-jin Wang, Chen-chen Zheng, Run-qi Fang, Xiu-min Hua, Yi-xuan Ni, Xiao-xia Jin, Hui Xia, Qiang Fang
• Format: Article in Journal/Newspaper
• Language: English
• Published: Hindawi Limited 2022
• Subjects: Arctic medicine. Tropical medicine; RC955-962; Arctic
• Online Access: https://doi.org/10.1155/2022/5715436; https://doaj.org/article/dd76c25e5dbe4d28ac4dc31f70e26d25

Dried blood spot (DBS) based PCR was considered an inexpensive and feasible method for detecting pathogens in the blood. The DBS carrier filter paper and PCR kits are crucial for accurate diagnosis. We evaluated 4 types of filter papers and 20 PCR kits for DBS samples. The PCR detecting Plasmodium results showed that the minimum detection limit of the 4 filter papers was 1 × 102 parasites/μL, and the positive rates of 20 PCR kits ranged from 0% to 100%. PCR results were satisfactory for detecting Plasmodium falciparum (P. falciparum) and Plasmodium. vivax (P. vivax) in archived DBS samples and Babesia gibsoni (B. gibsoni) in fresh pet DBS samples. Our results provided a useful reference for the detection of blood pathogens with DBS samples and direct PCR, especially for screening the cost-efficacy combination of filter paper and PCR kit in resource-limited areas.