The Comparison of PCR Kits for the Detection of Erythrocytic Parasites on Filter Paper

Dried blood spot (DBS) based PCR was considered an inexpensive and feasible method for detecting pathogens in the blood. The DBS carrier filter paper and PCR kits are crucial for accurate diagnosis. We evaluated 4 types of filter papers and 20 PCR kits for DBS samples. The PCR detecting Plasmodium r...

Full description

Bibliographic Details
Published in:Journal of Tropical Medicine
Main Authors: Zhi-yong Tao, Pei-yi Zhang, Lu Zhang, Chun-cao Li, Rui Hu, Han-wu Zhu, Bei Zhou, Kai Wu, Ling-xu Li, Da-wei Yao, Yu-jie Cao, Dao-jin Wang, Chen-chen Zheng, Run-qi Fang, Xiu-min Hua, Yi-xuan Ni, Xiao-xia Jin, Hui Xia, Qiang Fang
Format: Article in Journal/Newspaper
Language:English
Published: Hindawi Limited 2022
Subjects:
Arctic medicine. Tropical medicine
RC955-962
Arctic
Online Access:https://doi.org/10.1155/2022/5715436
https://doaj.org/article/dd76c25e5dbe4d28ac4dc31f70e26d25
Description
Summary:Dried blood spot (DBS) based PCR was considered an inexpensive and feasible method for detecting pathogens in the blood. The DBS carrier filter paper and PCR kits are crucial for accurate diagnosis. We evaluated 4 types of filter papers and 20 PCR kits for DBS samples. The PCR detecting Plasmodium results showed that the minimum detection limit of the 4 filter papers was 1 × 102 parasites/μL, and the positive rates of 20 PCR kits ranged from 0% to 100%. PCR results were satisfactory for detecting Plasmodium falciparum (P. falciparum) and Plasmodium. vivax (P. vivax) in archived DBS samples and Babesia gibsoni (B. gibsoni) in fresh pet DBS samples. Our results provided a useful reference for the detection of blood pathogens with DBS samples and direct PCR, especially for screening the cost-efficacy combination of filter paper and PCR kit in resource-limited areas.

Similar Items