Induction to tetraploidy in Pacific oysters (Crassostrea gigas)

As an alternative to the use of cytochalasin B (CB), 6-dimethylamino-purine (6-DMAP) and thermal shock (heat shock by increasing the temperature from 25 to 36ºC) could be used to induce tetraploidy in Pacific oyster (Crassostrea gigas) diploids. Induction was performed by applying shocks after elimi...

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Bibliographic Details
Published in:Acta Scientiarum. Animal Sciences
Main Authors: Emílio Mateus Costa Melo, Simone Sühnel, Francisco Carlos da Silva, Claudio Manoel Rodrigues de Melo
Format: Article in Journal/Newspaper
Language:English
Published: Editora da Universidade Estadual de Maringá (Eduem) 2022
Subjects:
Polyploidy
pacific oysters
reproduction
biotechnology
genetic enhancement
Animal culture
SF1-1100
Pacific
Crassostrea gigas
Pacific oyster
Online Access:https://doi.org/10.4025/actascianimsci.v44i1.55337
https://doaj.org/article/db8b29ab4a424f268379675bcb139756
Description
Summary:As an alternative to the use of cytochalasin B (CB), 6-dimethylamino-purine (6-DMAP) and thermal shock (heat shock by increasing the temperature from 25 to 36ºC) could be used to induce tetraploidy in Pacific oyster (Crassostrea gigas) diploids. Induction was performed by applying shocks after elimination of the first polar corpuscle at the end of meiosis I. Ploidy rates were verified using flow cytometry. Tetraploid larvae were obtained using all inductor (6-DMAP, thermal shock and CB) treatments. No difference in the efficiency of tetraploidy induction was noted among 6-DMAP, thermal shock and CB. The number of D-larvae and their yield, determined by calculating the percentage of well-formed D-larvae in relation to the total number of larvae, was similar (p > 0.05) among the evaluated induction methods. We suggest that 6-DMAP and thermal shock should be used in tetraploidy induction protocols, thereby avoiding the use of CB, which is a harmful agent for both humans and the environment.

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