Estimation of biodiversity and population structure of Russian reindeer (Rangifer tarandus) breeds inhabiting Northeastern Siberia (Republic of Sakha - Yakutia) using microsatellite markers

Three semi-domesticated reindeer breeds inhabiting the Republic of Sakha – Yakutia have been characterized using nine microsatellite markers. Genomic DNA was isolated from tissue samples of 123 individuals of the Chukotka (Khargin) (CHU, n=47), the Evenk (EVK, n=32) and the Even (EVN, n=44) breeds,...

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Bibliographic Details
Published in:Acta fytotechnica et zootechnica
Main Authors: Veronika Ruslanovna Kharzinova, Arsen Vladimirovich Dotsev, Anastasia Dmitrievna Solovieva, Valeriy Ivanovich Fedorov, Gottfried Brem, Natalia Anatolievna Zinovieva
Format: Article in Journal/Newspaper
Language:English
Published: Slovak University of Agriculture in Nitra 2016
Subjects:
FISH
reindeer breeds
genetic diversity
population structure
Agriculture
S
Agriculture (General)
S1-972
Sakha
Chukotka
Evenk
Rangifer tarandus
Republic of Sakha
Yakutia
Siberia
Online Access:https://doi.org/10.15414/afz.2016.19.03.87-92
https://doaj.org/article/d8a41c7cd7da468aa8daff6a9d76366f
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Summary:Three semi-domesticated reindeer breeds inhabiting the Republic of Sakha – Yakutia have been characterized using nine microsatellite markers. Genomic DNA was isolated from tissue samples of 123 individuals of the Chukotka (Khargin) (CHU, n=47), the Evenk (EVK, n=32) and the Even (EVN, n=44) breeds, collected from different regions of Yakutia. Fragment analysis and sizing were run on ABI 3131xl genetic analyzer. Allele frequencies were calculated and used for the characterization of reindeer breeds and the evaluation of their genetic biodiversity. Nei’s standard genetic distance was calculated and used for the construction of a neighbor-joining tree. Statistical analysis was conducted with GenAIEx 6.5.1, PAST2.15 and STRUCTURE2.3.4 software. The highest number of alleles, such as informative (with a frequency more than 5%), effective (Ne) and private (Pr), was detected in the CHU breed: Na≥5%=5.333±0.441, Ne=4.517±0393 and Pr =1.111±0.389, while the EVN breed had the lowest number: 4.778±0.324, 4.315±0.488 and 0.444±0.242, respectively. The EVN breed occupied an intermediate position (5.000±0.373, 4.408±0.315 and 0.889±0.261). Among reindeer breeds, observed heterozygosity ranged from 0.729 ± 0.026 to 0.608±0.050 with the lowest value found in CHU reindeer and the highest in EVK reindeer. A heterozygotes’ deficiency was observed in all reindeer breeds. At K=3, STRUCTURE analysis matches with the data of Nei's genetic distance dimension results, indicating the presence of a common consistent pattern. CHU and EVK reindeer breeds are characterized by a closer genetic relationship in comparison with the EVN breed, which formed a separate cluster.

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