Enzymatic modification of phosphatidylcholine with n-3 PUFA from silkworm oil fatty acids

α-Linolenic acid (ALA) containing phosphatidylcholine (PC) was prepared by an enzymatic method employing natural substrates comprising of egg and eri silkworm oil. Eri silkworm oil extracted from eri pupae was saponified to obtain the fatty acid mixture which was further subjected to urea complexati...

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Bibliographic Details
Published in:Grasas y Aceites
Main Authors: Shiva Shanker Kaki, T. Ravinder, B. Ashwini, B. V.S.K. Rao, R. B.N. Prasad
Format: Article in Journal/Newspaper
Language:English
Spanish
Published: Consejo Superior de Investigaciones Científicas 2014
Subjects:
α-linolenic acid
egg phosphatidylcholine
lipase
nutraceuticals
silkworm oil
Nutrition. Foods and food supply
TX341-641
Antarc*
Antarctica
Online Access:https://doi.org/10.3989/gya.097213
https://doaj.org/article/d6ec9012ec134944989b1d83e2453895
Description
Summary:α-Linolenic acid (ALA) containing phosphatidylcholine (PC) was prepared by an enzymatic method employing natural substrates comprising of egg and eri silkworm oil. Eri silkworm oil extracted from eri pupae was saponified to obtain the fatty acid mixture which was further subjected to urea complexation to obtain an ALA rich fraction with a purity of about 93%. Transesterification of PC with the ALA rich fraction with three immobilized lipases namely Lipozyme TL IM, Lipozyme RM IM and lipase from Candida Antarcticaz showed that only the lipase from Candida antarctica was successful for the incorporation of ALA into egg yolk PC. It was found that ALA was incorporated by up to 27% in the sn-1 position of egg PC and the positional distribution analysis of fatty acids in the modified PC showed that the sn-1 position was found to contain about 59% ALA.

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