Bacteriophage Cocktail for the Prevention of Multiple-Antibiotic-Resistant and Mono-Phage-Resistant Vibrio coralliilyticus Infection in Pacific Oyster ( Crassostrea gigas ) Larvae

Vibrio coralliilyticus ( V. coralliilyticus ) is a pathogen that causes mass mortality in marine bivalve hatcheries worldwide. In this study, we used a bacteriophage (phage) cocktail to prevent multiple-antibiotic-resistant (MAR) and phage-resistant (PR) V. coralliilyticus infection in Pacific oyste...

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Bibliographic Details
Published in:Pathogens
Main Authors: Hyoun Joong Kim, Jin Woo Jun, Sib Sankar Giri, Sang Guen Kim, Sang Wha Kim, Jun Kwon, Sung Bin Lee, Cheng Chi, Se Chang Park
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2020
Subjects:
pacific oyster larvae
mass mortality
Vibrio coralliilyticus
bacteriophage
phage- resistant variant
cocktail phage
Medicine
R
Pacific
Crassostrea gigas
Pacific oyster
Online Access:https://doi.org/10.3390/pathogens9100831
https://doaj.org/article/d49b4e70b18e44499b7ac582abd247e0
Description
Summary:Vibrio coralliilyticus ( V. coralliilyticus ) is a pathogen that causes mass mortality in marine bivalve hatcheries worldwide. In this study, we used a bacteriophage (phage) cocktail to prevent multiple-antibiotic-resistant (MAR) and phage-resistant (PR) V. coralliilyticus infection in Pacific oyster ( Crassostrea gigas ) larvae. To prevent the occurrence of phage-resistant strains and decrease the effect of mono-phage treatment, we prepared a phage cocktail containing three types of V. coralliilyticus -specific phages and tested its prophylactic efficacy against MAR and PR V. coralliilyticus infection. The results of the cell lysis test showed that the phage cocktail showed an excellent bactericidal effect against the MAR and PR variants in contrast to the experimental group treated with two mono phages (pVco-5 and pVco-7). An in vivo test using Pacific oyster larvae also confirmed the preventive effect against MAR and PR variants. In conclusion, the application of the phage cocktail effectively prevented V. coralliilyticus infection in marine bivalve seedling production. Furthermore, it is expected to reduce damage to the aquaculture industry caused by the occurrence of MAR and PR V. coralliilyticus . Therefore, phage cocktails may be used for the control of various bacterial diseases.

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