Evaluación de la ingesta de colesterol en conejos infectados con Trypanosoma cruzi

• Main Authors: Alarcón M, Añez N, Calderón L, Matousek A
• Format: Article in Journal/Newspaper
• Language: English; Spanish
• Published: Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales 2004
• Subjects: Infection; Trypanosoma cruzi; rabbits; cholesterol; high-density lipoproteins; low-density lipoproteins; Infección; conejos; colesterol; lipoproteínas de alta densidad; lipoproteínas de baja densidad; Arctic medicine. Tropical medicine; RC955-962; Public aspects of medicine; RA1-1270; Alta; Arctic; Baja
• Online Access: https://doaj.org/article/d366e983f2f9443e97f1315c0d7cab99

Abstract: In order to determine the possible effect of Trypanosoma cruzi infection and an atherogenic diet on the atherosclerosis-Chagas interaction in rabbits (Sylvilagus floridanus orinoci), a study was made. The animals were divided into four groups: I.- healthy control; II.- atherogenic (Colesterol 60mg/day) diet; III.- atherogenic diet infected with Y strain subcutaneously (2x105 bloodstream trypomastigotes injected subcutaneously), and IV infected with T. cruzi infection. Total Cholesterol concentration (TC), low density (LDL) and high-density lipoprotein (HDL) levels were measured in plasma using microenzymatic colorimetric analysis and analysis of variance (ANOVA) was applied to the results. The atherogenic diet produced a progressive increase of TC, LDL and HDL in groups II and III compared with the healthy and infected groups. TC, HDL and LDL levels were significantly lower (P 0.05) in group III with respect to group II rabbits. The reduction of TC suggests a diminished capacity of infected liver for lipoproteins to liberate and/or a greater peroxidation of HDL and LDL as an effect of the infection, with a consequent increase in cholesterol bile elimination. Finally, the results suggest that cholesterol diet and T. cruzi interaction could favor atherosclerosis disease. On the other hand, the endothelial damage caused by these parasites could favor the cholesterol deposit in the microvasculature and at the same time the lower HDL levels, produce an increase of the atherogenicity through a diet high in cholesterol. Resumen: Con el propósito de determinar el posible efecto de la infección por Trypanosoma cruzi y una dieta aterogénica sobre la interacción Chagas- ateroesclerosis se evaluaron grupos de conejos Silvilagus floridanus orinoci: I control sano II con dieta aterogénica (colesterol 60 mg/día); III dieta aterogénica e infectados con 2x105 tripomastigotos sanguícolas cepa “Y” vía subcutánea y IV infectados con T. cruzi. Los niveles de colesterol total (CT), lipoproteínas de baja densidad (LDL) y ...