Purification, Chemical Characterization and Immunomodulatory Activity of a Sulfated Polysaccharide from Marine Brown Algae Durvillaea antarctica
An immunomodulatory polysaccharide (DAP4) was extracted, purified, and characterized from Durvillaea antarctica. The results of chemical and spectroscopic analyses demonstrated that the polysaccharide was a fucoidan, and was mainly composed of (1→3)-α- l -Fuc p and (1→4)-α- l -Fuc p residues with a...
Published in: | Marine Drugs |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
MDPI AG
2022
|
Subjects: | |
Online Access: | https://doi.org/10.3390/md20040223 https://doaj.org/article/d1f3d9ade57a4f2385103ae9db28a0d5 |
id |
ftdoajarticles:oai:doaj.org/article:d1f3d9ade57a4f2385103ae9db28a0d5 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:d1f3d9ade57a4f2385103ae9db28a0d5 2023-05-15T13:57:17+02:00 Purification, Chemical Characterization and Immunomodulatory Activity of a Sulfated Polysaccharide from Marine Brown Algae Durvillaea antarctica Ling Qin Hui Xu Yingying He Chen Liang Kai Wang Junhan Cao Changfeng Qu Jinlai Miao 2022-03-01T00:00:00Z https://doi.org/10.3390/md20040223 https://doaj.org/article/d1f3d9ade57a4f2385103ae9db28a0d5 EN eng MDPI AG https://www.mdpi.com/1660-3397/20/4/223 https://doaj.org/toc/1660-3397 doi:10.3390/md20040223 1660-3397 https://doaj.org/article/d1f3d9ade57a4f2385103ae9db28a0d5 Marine Drugs, Vol 20, Iss 223, p 223 (2022) Durvillaea antarctica fucoidan structural characterization immunomodulatory Biology (General) QH301-705.5 article 2022 ftdoajarticles https://doi.org/10.3390/md20040223 2022-12-30T20:39:41Z An immunomodulatory polysaccharide (DAP4) was extracted, purified, and characterized from Durvillaea antarctica. The results of chemical and spectroscopic analyses demonstrated that the polysaccharide was a fucoidan, and was mainly composed of (1→3)-α- l -Fuc p and (1→4)-α- l -Fuc p residues with a small degree of branching at C-3 of (1→4)-α- l -Fuc p residues. Sulfate groups were at C-4 of (1→3)-α- l -Fuc p , C-2 of (1→4)-α- l -Fuc p and minor C-6 of (1→4)-β- d -Gal p . Small amounts of xylose and galactose exist in the forms of β- d -Xyl p -(1→ and β- d -Gal-(1→. The immunomodulatory activity of DAP4 was measured on RAW 264.7 cells, the results proved that DAP4 exhibited excellent immunomodulatory activities, such as promoted the proliferation of spleen lymphocytes, increased NO production, as well as enhanced phagocytic of macrophages. Besides, DAP4 could also produce better enhancement on the vitality of NK cells. For the high immunomodulatory activity, DAP4 might be a potential source of immunomodulatory fucoidan with a novel structure. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Marine Drugs 20 4 223 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Durvillaea antarctica fucoidan structural characterization immunomodulatory Biology (General) QH301-705.5 |
spellingShingle |
Durvillaea antarctica fucoidan structural characterization immunomodulatory Biology (General) QH301-705.5 Ling Qin Hui Xu Yingying He Chen Liang Kai Wang Junhan Cao Changfeng Qu Jinlai Miao Purification, Chemical Characterization and Immunomodulatory Activity of a Sulfated Polysaccharide from Marine Brown Algae Durvillaea antarctica |
topic_facet |
Durvillaea antarctica fucoidan structural characterization immunomodulatory Biology (General) QH301-705.5 |
description |
An immunomodulatory polysaccharide (DAP4) was extracted, purified, and characterized from Durvillaea antarctica. The results of chemical and spectroscopic analyses demonstrated that the polysaccharide was a fucoidan, and was mainly composed of (1→3)-α- l -Fuc p and (1→4)-α- l -Fuc p residues with a small degree of branching at C-3 of (1→4)-α- l -Fuc p residues. Sulfate groups were at C-4 of (1→3)-α- l -Fuc p , C-2 of (1→4)-α- l -Fuc p and minor C-6 of (1→4)-β- d -Gal p . Small amounts of xylose and galactose exist in the forms of β- d -Xyl p -(1→ and β- d -Gal-(1→. The immunomodulatory activity of DAP4 was measured on RAW 264.7 cells, the results proved that DAP4 exhibited excellent immunomodulatory activities, such as promoted the proliferation of spleen lymphocytes, increased NO production, as well as enhanced phagocytic of macrophages. Besides, DAP4 could also produce better enhancement on the vitality of NK cells. For the high immunomodulatory activity, DAP4 might be a potential source of immunomodulatory fucoidan with a novel structure. |
format |
Article in Journal/Newspaper |
author |
Ling Qin Hui Xu Yingying He Chen Liang Kai Wang Junhan Cao Changfeng Qu Jinlai Miao |
author_facet |
Ling Qin Hui Xu Yingying He Chen Liang Kai Wang Junhan Cao Changfeng Qu Jinlai Miao |
author_sort |
Ling Qin |
title |
Purification, Chemical Characterization and Immunomodulatory Activity of a Sulfated Polysaccharide from Marine Brown Algae Durvillaea antarctica |
title_short |
Purification, Chemical Characterization and Immunomodulatory Activity of a Sulfated Polysaccharide from Marine Brown Algae Durvillaea antarctica |
title_full |
Purification, Chemical Characterization and Immunomodulatory Activity of a Sulfated Polysaccharide from Marine Brown Algae Durvillaea antarctica |
title_fullStr |
Purification, Chemical Characterization and Immunomodulatory Activity of a Sulfated Polysaccharide from Marine Brown Algae Durvillaea antarctica |
title_full_unstemmed |
Purification, Chemical Characterization and Immunomodulatory Activity of a Sulfated Polysaccharide from Marine Brown Algae Durvillaea antarctica |
title_sort |
purification, chemical characterization and immunomodulatory activity of a sulfated polysaccharide from marine brown algae durvillaea antarctica |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/md20040223 https://doaj.org/article/d1f3d9ade57a4f2385103ae9db28a0d5 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Marine Drugs, Vol 20, Iss 223, p 223 (2022) |
op_relation |
https://www.mdpi.com/1660-3397/20/4/223 https://doaj.org/toc/1660-3397 doi:10.3390/md20040223 1660-3397 https://doaj.org/article/d1f3d9ade57a4f2385103ae9db28a0d5 |
op_doi |
https://doi.org/10.3390/md20040223 |
container_title |
Marine Drugs |
container_volume |
20 |
container_issue |
4 |
container_start_page |
223 |
_version_ |
1766264880831135744 |