Purification, Chemical Characterization and Immunomodulatory Activity of a Sulfated Polysaccharide from Marine Brown Algae Durvillaea antarctica
An immunomodulatory polysaccharide (DAP4) was extracted, purified, and characterized from Durvillaea antarctica. The results of chemical and spectroscopic analyses demonstrated that the polysaccharide was a fucoidan, and was mainly composed of (1→3)-α- l -Fuc p and (1→4)-α- l -Fuc p residues with a...
| Published in: | Marine Drugs |
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| Main Authors: | , , , , , , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
MDPI AG
2022
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| Subjects: | |
| Online Access: | https://doi.org/10.3390/md20040223 https://doaj.org/article/d1f3d9ade57a4f2385103ae9db28a0d5 |
| Summary: | An immunomodulatory polysaccharide (DAP4) was extracted, purified, and characterized from Durvillaea antarctica. The results of chemical and spectroscopic analyses demonstrated that the polysaccharide was a fucoidan, and was mainly composed of (1→3)-α- l -Fuc p and (1→4)-α- l -Fuc p residues with a small degree of branching at C-3 of (1→4)-α- l -Fuc p residues. Sulfate groups were at C-4 of (1→3)-α- l -Fuc p , C-2 of (1→4)-α- l -Fuc p and minor C-6 of (1→4)-β- d -Gal p . Small amounts of xylose and galactose exist in the forms of β- d -Xyl p -(1→ and β- d -Gal-(1→. The immunomodulatory activity of DAP4 was measured on RAW 264.7 cells, the results proved that DAP4 exhibited excellent immunomodulatory activities, such as promoted the proliferation of spleen lymphocytes, increased NO production, as well as enhanced phagocytic of macrophages. Besides, DAP4 could also produce better enhancement on the vitality of NK cells. For the high immunomodulatory activity, DAP4 might be a potential source of immunomodulatory fucoidan with a novel structure. |
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