Determination of fluoride in Antarctic krill (Euphausia superba) using ion chromatography and its pretreatments selection

A rapid, sensitive and reliable method to quantify fluoride in Antarctic krill has been established. Four different pretreatment methods were used for the extraction of fluoride: double-deionised water extraction, sulphuric acid distillation, hydrochloric acid extraction, and pH adjustment with buff...

Full description

Bibliographic Details
Published in:Czech Journal of Food Sciences
Main Authors: Yan-Ling ZHAO, Lan-Lan ZHU, Yong SUN, De-Qing ZHOU
Format: Article in Journal/Newspaper
Language:English
Published: Czech Academy of Agricultural Sciences 2015
Subjects:
S
Online Access:https://doi.org/10.17221/498/2013-CJFS
https://doaj.org/article/d12bf2e6c6f142d793490f3468e37c1c
Description
Summary:A rapid, sensitive and reliable method to quantify fluoride in Antarctic krill has been established. Four different pretreatment methods were used for the extraction of fluoride: double-deionised water extraction, sulphuric acid distillation, hydrochloric acid extraction, and pH adjustment with buffer after hydrochloric acid extraction. Four methods of comparative analysis revealed that sulphuric acid distillation was suitable preparation for ion chromatography determination of fluoride in Antarctic krill (fluoride content 288.7 ± 10.2 mg/kg). The method was partially validated in linearity, accuracy, and precision. The linear range was from 0.1 to 10.0 mg/l with the regression coefficient of 0.99998. The accuracy expressed as the recoveries of standard addition ranged from 95.3% to 101.3%, the relative standard deviation (n = 8) was 1.8-1.9%. With this method, the 3σ limit of detection was 0.06 mg/l of fluoride in Antarctic krill. Our results indicate that the method (limit of quantification 0.2 mg/l) could be well applied for the determination of fluoride in Antarctic krill.